A novel strategy forin vivoangiogenesis and osteogenesis: magnetic micro-movement in a bone scaffold
| Autor: | Xiaolan Yang, Changxuan Wang, Ming Li, Yujia Luo, Xin Zhang, Chaode Cen, Quan Kang, Dianming Jiang, Hong An, Xiao-Bo Zhang, Cong Luo, Hua Huang, Hui Hui, Tong-Chuan He, Lina Xie, Tingyu Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Scaffold Angiogenesis VEGF receptors Biomedical Engineering Neovascularization Physiologic Pharmaceutical Science Medicine (miscellaneous) 02 engineering and technology Gene delivery Transfection Bone and Bones Motion 03 medical and health sciences Osteogenesis In vivo Animals Magnetite Nanoparticles Chitosan Drug Carriers Tissue Engineering Tissue Scaffolds biology Chemistry Bone scaffold General Medicine 021001 nanoscience & nanotechnology Cell biology Drug Liberation Magnetic Fields 030104 developmental biology Key factors biology.protein Rabbits 0210 nano-technology Plasmids Biotechnology |
| Zdroj: | Artificial Cells, Nanomedicine, and Biotechnology. 46:636-645 |
| ISSN: | 2169-141X 2169-1401 |
| DOI: | 10.1080/21691401.2018.1465947 |
| Popis: | Angiogenesis and osteogenesis in tissue-engineered bone are the key factors in the clinical application of tissue-engineering technology to repair large bone defects. In vivo cells that are farther than 200 μm from capillaries cannot survive due to lack of nutrients and oxygen, and thus, the tissue-engineered bone is not suitable for repairing large bone defects. In this study, we constructed a novel artificial bone scaffold loaded with superparamagnetic plasmid gene microspheres. Magnetic micro-movement of the magnetic microspheres in the scaffold was generated by an oscillating magnetic field and a static magnetic field to promote the release of plasmid genes from microspheres for transfection of surrounding cells, resulting in protein expression of vascular endothelial growth factor, thus promoting angiogenesis and osteogenesis in the scaffold, internal vascularization of the artificial bone scaffold and repair of large bone defects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |