Deletion mapping of the polA-metB region of the Escherichia coli chromosome
| Autor: | Bonnie Tyler, G Pahel, F R Bloom |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Transposable element
Genetic Linkage Locus (genetics) Biology medicine.disease_cause Rhamnose Microbiology Glutamate-Ammonia Ligase Transduction Genetic Genetic linkage Escherichia coli medicine Tn10 Deletion mapping Molecular Biology Gene Prophage Recombination Genetic Genetics Chromosome Mapping Molecular biology Genes Mutation bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 138:653-656 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.138.2.653-656.1979 |
| Popis: | A lambdacI857 prophage inserted into one of the genes of the rha locus was used to select deletions unambiguously ordering the markers polA-glnA-rha-pfkA-tpi-metBJF. Transduction with phage P1 indicates at least 70% linkage between glnA and polA. The order of the pfk and tpi markers is reversed from that previously published. Despite the relatively large distance separating the glnA and rha loci, deletions removing this entire region have no obvious phenotype. The isolation of Tn10 transposons integrated at different sites between rha and glnA greatly facilitated this work. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|