Complex interactions amongst N-cadherin, DLAR, and Liprin-α regulate Drosophila photoreceptor axon targeting
| Autor: | Katharine M. H. Tomalty, Aurnab Ghose, Helen M. McLendon, Saurabh Prakash, David Van Vactor, Thomas R. Clandinin, Kelsey L. Clark, Catherine Irene Dubreuil, Kelly A. Dennehy, Jennifer J. Hwa |
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| Rok vydání: | 2009 |
| Předmět: |
Embryo
Nonmammalian Neurodevelopment Green Fluorescent Proteins Receptor-Like Protein Tyrosine Phosphatases Target selection Context (language use) Protein tyrosine phosphatase Biology Axon Article Animals Drosophila Proteins Immunoprecipitation Cell adhesion Growth cone Molecular Biology Photoreceptor Synapse assembly Cadherin Intracellular Signaling Peptides and Proteins Cell Biology Cadherins Phosphoproteins Synapse Axons Cell biology Drosophila melanogaster Synapses Drosophila Photoreceptor Cells Invertebrate Drosophila Protein Protein Binding Developmental Biology |
| Zdroj: | Developmental Biology. 336:10-19 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2009.09.016 |
| Popis: | The formation of stable adhesive contacts between pre- and post-synaptic neurons represents the initial step in synapse assembly. The cell adhesion molecule N-cadherin, the receptor tyrosine phosphatase DLAR, and the scaffolding molecule Liprin-alpha play critical, evolutionarily conserved roles in this process. However, how these proteins signal to the growth cone and are themselves regulated remains poorly understood. Using Drosophila photoreceptors (R cells) as a model, we evaluate genetic and physical interactions among these three proteins. We demonstrate that DLAR function in this context is independent of phosphatase activity but requires interactions mediated by its intracellular domain. Genetic studies reveal both positive and, surprisingly, inhibitory interactions amongst all three genes. These observations are corroborated by biochemical studies demonstrating that DLAR physically associates via its phosphatase domain with N-cadherin in Drosophila embryos. Together, these data demonstrate that N-cadherin, DLAR, and Liprin-alpha function in a complex to regulate adhesive interactions between pre- and post-synaptic cells and provide a novel mechanism for controlling the activity of Liprin-alpha in the developing growth cone. |
| Databáze: | OpenAIRE |
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