Properties and characterization of low molecular weight inhibitor of calmodulin-dependent cAMP phosphodiesterase from rat liver
| Autor: | Solomon S. Solomon, R. Hanumantha Rao, J.A. Smoake, Laura Sanders, M. R. Palazzolo |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Calmodulin
medicine.medical_treatment Fractional Precipitation General Biochemistry Genetics and Molecular Biology Diabetes mellitus medicine Animals General Pharmacology Toxicology and Pharmaceutics Chromatography High Pressure Liquid Chromatography biology Insulin food and beverages Phosphodiesterase General Medicine Cyclic Nucleotide Phosphodiesterases Type 1 medicine.disease Rats Molecular Weight Liver Mechanism of action Biochemistry 3' 5'-Cyclic-AMP Phosphodiesterases Enzyme inhibitor Rat liver biology.protein medicine.symptom |
| Zdroj: | Life Sciences. 35:2155-2164 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(84)90516-2 |
| Popis: | We have identified two different species of inhibitors of calmodulin-dependent cAMP phosphodiesterase: 1) a low molecular weight (LMW) and 2) a high molecular weight (HMW) form. These inhibitors are extracted from rat liver. Both LMW and HMW inhibitors are heat-stable, acidic in nature and lose activity with prolonged storage and/or repeated freezing and thawing. The low molecular weight inhibitor has been purified to about 7,000-fold with 300% recovery. LMW inhibits calmodulin-dependent cAMP phosphodiesterase regardless of the source of calmodulin (e.g. fat, brain, heart, erythrocytes). LMW appears to be lipid in nature with a molecular weight of 1,500–5,000. The role of these inhibitors in diabetes and mechanism of action of insulin is presented. |
| Databáze: | OpenAIRE |
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