Magnetic Ultrathin Tissue Sections for Ease of Light and Electron Microscopy
| Autor: | Leite, Yamahachi H, Richard H. R. Hahnloser, Daniel Normen Düring, Gabor Csucs, Koestinger G, Rickauer S |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Materials science business.industry Scanning electron microscope Nanoparticle Substrate (electronics) Fluorescence law.invention 03 medical and health sciences 0302 clinical medicine Tissue sections law Magnet Optoelectronics Wafer Electron microscope business 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | bioRxiv |
| DOI: | 10.1101/532549 |
| Popis: | Electron microscopy (EM) tends to be a tedious imaging technique that requires a diverse manual skill set, in particular when it comes to collecting and processing large numbers of consecutive ultrathin sections of biological tissue. To alleviate the challenges of collecting freshly cut sections floating on the water surface of an ultramicrotome, resins can be enhanced with nanoparticles to allow for accumulation of ultrathin sections under a magnet. Subsequent water removal deposits the dense section conglomerate onto an immersed EM imaging substrate. We describe an application of this workflow, termed Magnetic Section Collection (MagC), on ultrathin sections that were deposited on a silicon wafer and imaged in both light and scanning electron microscopes. We show that fluorescence data from three types of retrograde neuronal tracers are highly informative about projection neuron type and we propose a simple approach for neuron identification based on counting the percentage of consistently colored pixels across adjacent ultrathin sections. |
| Databáze: | OpenAIRE |
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