Plasminogen activator inhibitor-1 (PAI-1) stimulates human corneal epithelial cell adhesion and migration in vitro
Autor: | Zhiyu Wang, Michelle Kurpakus-Wheater, Gabriel Sosne |
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Rok vydání: | 2005 |
Předmět: |
Serine Proteinase Inhibitors
Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Cell Movement Cornea Plasminogen Activator Inhibitor 1 Cell Adhesion medicine Humans Eye Proteins Cells Cultured Corneal epithelium Chemotaxis Epithelium Corneal Epithelial Cells Adhesion Molecular biology eye diseases Sensory Systems Epithelium Ophthalmology medicine.anatomical_structure Microscopy Fluorescence chemistry Plasminogen activator inhibitor-1 Laminin sense organs Wound healing Plasminogen activator |
Zdroj: | Experimental Eye Research. 80:1-8 |
ISSN: | 0014-4835 |
DOI: | 10.1016/j.exer.2004.06.006 |
Popis: | In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this study was to extend those observations by determining the effect of exogenous PAI-1 on the migration and adhesion of human corneal epithelial cells (HCEC) in vitro. The expression of PAI-1 in non-transformed early passage HCEC was confirmed by immunofluorescence microscopy and Western blot analysis. Colorimetric assays coupled with function-inhibiting antibody studies using the matrix assembled in situ by cultured cells demonstrate that immobilized PAI-1 serves as an efficient substrate for HCEC adhesion. HCEC attachment to PAI-1 is comparable to that of laminin-10, a known strong adhesion protein for epithelial cells. In addition to serving as an adhesion substrate, PAI-1 also functions as a chemotactic agent for corneal epithelium. Additionally it promotes the random migration of HCEC, from an initial cell cluster, along a culture substrate. Our results in corneal epithelium are consistent with reports from other investigators showing that PAI-1 facilitates both epithelial adhesion and migration. From our studies we conclude that PAI-1 may play a dual role in corneal wound healing. Initially PAI-1 may function to stimulate migration and facilitate the reepithelialization of the wound bed. Post-reepithelization, PAI-1 may ensure corneal epithelial cell adhesion to matrix to promote successful wound healing. |
Databáze: | OpenAIRE |
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