Probing molecular pathways for DNA orientational trapping, unzipping and translocation in nanopores by using a tunable overhang sensor
| Autor: | Lehr L. Hunter, Yong Wang, Brandon Ritzo, Kai Tian, Li-Qun Gu |
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| Rok vydání: | 2014 |
| Předmět: |
Models
Molecular Materials science Nanotechnology Equipment Design Materials testing Trapping Article Equipment Failure Analysis Motion Nanopore chemistry.chemical_compound Models Chemical chemistry Materials Testing Nucleic acid Biophysics Computer-Aided Design Nucleic Acid Conformation Computer Simulation General Materials Science DNA Probes DNA Oligonucleotide Array Sequence Analysis Nucleic acid detection |
| Zdroj: | Nanoscale. 6:11372-11379 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c4nr03195d |
| Popis: | Nanopores provide a unique single-molecule platform for genetic and epigenetic detection. The target nucleic acids can be accurately analyzed by characterizing their specific electric fingerprints or signatures in the nanopore. Here we report a series of novel nanopore signatures generated by target nucleic acids that are hybridized with a probe. A length-tunable overhang appended to the probe functions as a sensor to specifically modulate the nanopore current profile. The resulting signatures can reveal multiple mechanisms for the orientational trapping, unzipping, escaping and translocation of nucleic acids in the nanopore. This universal approach can be used to program various molecular movement pathways, elucidate their kinetics, and enhance the sensitivity and specificity of the nanopore sensor for nucleic acid detection. |
| Databáze: | OpenAIRE |
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