Increased EHEC survival and virulence gene expression indicate an enhanced pathogenicity upon simulated pediatric gastrointestinal conditions
| Autor: | Charlène Roussel, Jonathan Thévenot, Delphine Thevenot-Sergentet, Charlotte Cordonnier, Olivier Le Goff, Valérie Livrelli, Wessam Galia, Sandrine Chalancon, Françoise Leriche, Monique Alric, Tom Van de Wiele, Stéphanie Blanquet-Diot |
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| Přispěvatelé: | Microbiologie Environnement Digestif Santé (MEDIS), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), CALITYSS, Typicité des produits alimentaires (CALITYSS), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Virulence Factors [SDV]Life Sciences [q-bio] 030106 microbiology Fimbria Virulence medicine.disease_cause Escherichia coli O157 Models Biological Microbiology Shiga Toxin Pathogenesis 03 medical and health sciences Intestine Small medicine Humans Adhesins Bacterial Child Escherichia coli Gene Escherichia coli Infections Intimin biology Shiga toxin Flow Cytometry Virology 3. Good health Bacterial adhesin Kinetics Gastric Mucosa Enterohemorrhagic Escherichia coli Pediatrics Perinatology and Child Health biology.protein |
| Zdroj: | Pediatric Research Pediatric Research, Nature Publishing Group, 2016, 80 (5), pp.734-743. ⟨10.1038/pr.2016.144⟩ |
| ISSN: | 0031-3998 1530-0447 |
| DOI: | 10.1038/pr.2016.144⟩ |
| Popis: | Enterohemorrhagic Escherichia coli (EHEC) are major foodborne pathogens that constitute a serious public health threat, mainly in young children. Shiga toxins (Stx) are the main virulence determinants of EHEC pathogenesis but adhesins like intimin (eae) and Long polar fimbriae (Lpf) also contribute to infection. The TNO GastroIntestinal Model (TIM) was used for a comparative study of EHEC O157:H7 survival and virulence under adult and child digestive conditions. Survival kinetics in the in vitro digestive tract were determined by plating while bacterial viability was assessed by flow cytometry analysis. Expression of stx, eae, and lpf genes was followed by reverse transcriptase-quantitative PCR (RT-qPCR) and Stx production was measured by ELISA (enzyme-linked immunosorbent assay). Upon gastrointestinal passage, a higher amount of viable cells was found in the simulated ileal effluents of children compared to that of adults (with 34 and 6% of viable cells, respectively). Expression levels of virulence genes were up to 125-fold higher in children. Stx was detected only in child ileal effluents. Differences in digestive physicochemical parameters may partially explain why children are more susceptible to EHEC infection than adults. Such data are essential for a full understanding of EHEC pathogenesis and would help in designing novel therapeutic approaches. |
| Databáze: | OpenAIRE |
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