Involvement of cAMP in inhibition of maturation of follicle-enclosed oocytes by actinomycin D inXenopus laevis andRana temporaria
| Autor: | M. N. Skoblina, I. Huhtaniemi, Matikainen T |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Amphibian
medicine.medical_specialty Rana temporaria Xenopus Rana Xenopus laevis Follicle Oogenesis Ovarian Follicle biology.animal Internal medicine Cyclic AMP medicine Animals Nuclear function Estradiol biology Gonadotropic hormones General Medicine Oocyte biology.organism_classification medicine.anatomical_structure Endocrinology Dactinomycin Oocytes Female Animal Science and Zoology Hormone |
| Zdroj: | Journal of Experimental Zoology. 273:142-148 |
| ISSN: | 1097-010X 0022-104X |
| DOI: | 10.1002/jez.1402730207 |
| Popis: | Production of estradiol-17β and cAMP was assayed during maturation of the follicle-enclosed oocytes of Xenopus laevis and Rana temporaria stimulated by pituitary suspension or hCG, correspondingly. In some samples, the nuclear function was suppressed by pretreatment with AD. The level of estradiol-17β in the oocytes of the both species was very low and was not affected by hormonal or AD treatment, while the level of cAMP (follicle + medium) increased. When maturation was stimulated by low concentrations of gonadotropic hormones inducing complete or near 100% maturation, the cAMP level increased 1.4- to 3.9-fold. Both higher concentrations of the gonadotropic hormones and pretreatment of the follicles with AD increased the cAMP level still more. Combination of these treatments led to a greater increase of cAMP and to inhibition of oocyte maturation. We conclude that cAMP, rather than estradiol-17β, is involved in the AD-induced inhibition of amphibian oocyte maturation stimulated by gonadotropins. © 1995 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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