Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay: Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases
| Autor: | Marlene DeLeon-Carnes, Wun-Ju Shieh, Christopher D. Paddock, Zoi-Anna Antoniadou, Kakali Bandyopadhyay, Julu Bhatnagar, Kathryn L. Kellar, Sherif R. Zaki |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Adolescent Article Subject Clostridium perfringens Clostridium sordellii Dermatology medicine.disease_cause Polymerase Chain Reaction lcsh:Gynecology and obstetrics lcsh:Infectious and parasitic diseases law.invention Microbiology Clostridia Young Adult Clostridium Pregnancy law Humans Medicine lcsh:RC109-216 Pregnancy Complications Infectious lcsh:RG1-991 Polymerase chain reaction biology business.industry Obstetrics and Gynecology Toxic shock syndrome biology.organism_classification medicine.disease Shock Septic Microspheres Infectious Diseases Clostridium Infections Immunohistochemistry Female Endometritis business Research Article |
| Zdroj: | Infectious Diseases in Obstetrics and Gynecology, Vol 2012 (2012) Infectious Diseases in Obstetrics and Gynecology |
| ISSN: | 1064-7449 |
| DOI: | 10.1155/2012/972845 |
| Popis: | Clostridium sordelliiandClostridium perfringensare infrequent human pathogens; however, the case-fatality rates for the infections are very high, particularly in obstetricC. sordelliiinfections (>90%). Deaths fromClostridium sordelliiandClostridium perfringenstoxic shock (CTS) are sudden, and diagnosis is often challenging. Formalin-fixed, paraffin-embedded (FFPE) tissues usually are the only specimens available for sudden fatal cases, and immunohistochemistry (IHC) for Clostridia is generally performed but it cannot identify species. A clear need exists for a rapid, species-specific diagnostic assay for FFPE tissues. We developed a duplex PCR-based microsphere assay for simultaneous detection ofC. sordelliiandC. perfringensand evaluated DNA extracted from 42Clostridiumisolates and FFPE tissues of 28 patients with toxic shock/endometritis (20 CTS, 8 non-CTS, as confirmed by PCR and sequencing). The microsphere assay correctly identifiedC. sordelliiandC. perfringensin all known isolates and in all CTS patients (10C. sordellii, 8C. perfringens, 2 both) and showed 100% concordance with PCR and sequencing results. The microsphere assay is a rapid, specific, and cost-effective method for the diagnosis of CTS and offers the advantage of simultaneous testing forC. sordelliiandC. perfringensin FFPE tissues using a limited amount of DNA. |
| Databáze: | OpenAIRE |
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