Virulence gene profiles and intimin subtypes of Shiga toxin-producing Escherichia coli isolated from healthy and diarrhoeic calves
| Autor: | M. Askari Badouei, T. Zahraei Salehi, H. Tadjbakhsh, M. G. Nadalian, G. Nikbakht Brujeni, M. Rabbani Khorasgani |
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| Rok vydání: | 2010 |
| Předmět: |
Diarrhea
Serotype Virulence Factors Colony Count Microbial Cattle Diseases Virulence Biology Shiga Toxins medicine.disease_cause Polymerase Chain Reaction Microbiology law.invention fluids and secretions STX2 law medicine Animals Serotyping Escherichia coli Escherichia coli Infections Feces Polymerase chain reaction Intimin Shiga-Toxigenic Escherichia coli General Veterinary General Medicine bacterial infections and mycoses Virology Animals Newborn Case-Control Studies bacteria Cattle Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | Veterinary Record. 167:858-861 |
| ISSN: | 0042-4900 |
| DOI: | 10.1136/vr.c4009 |
| Popis: | The virulence properties of Shiga toxin-producing Escherichia coli (STEC) strains isolated from diarrhoeic and non-diarrhoeic calves were compared. The strains were also tested for O157:H7, O111 and O26 serotypes, using PCR and conventional serotyping methods. E coli strains isolated from 297 faecal samples, from 200 diarrhoeic and 97 non-diarrhoeic calves, were screened by multiplex PCR assay for the stx1, stx2, eae and Ehly virulence genes. STECs were recovered from 8 per cent of diarrhoeic calves and 10.3 per cent of non-diarrhoeic calves. The predominant virulence gene profile was stx1/eae/Ehly (47.3 per cent) among isolates from diarrhoeic calves and eae/Ehly (36.8 per cent) among isolates from non-diarrhoeic calves. Among three tested serogroups, the predominant serogroup was O26 (18.4 per cent), and O157:H7 was not detected. Intimin subtyping by restriction fragment length polymorphism analysis revealed only three intimin subtypes (β, γ and ). A significant difference was observed in the distribution of Int- between two groups. Int- was present in 50 per cent of the isolates from diarrhoeic calves and in 11.1 per cent of the isolates from non-diarrhoeic calves; this difference was statistically significant (P=0.01). |
| Databáze: | OpenAIRE |
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