Characterization of Poliovirus Conformational Alteration Mediated by Soluble Cell Receptors
| Autor: | A Gómez Yafal, Vincent R. Racaniello, G Kaplan, James M. Hogle |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Conformational change
viruses Moths Spodoptera Transfection medicine.disease_cause Cell Line Leucine Cell surface receptor Virology medicine Animals Receptor biology Poliovirus Osmolar Concentration Membrane Proteins Hydrogen-Ion Concentration biology.organism_classification Recombinant Proteins eye diseases Microscopy Electron Biochemistry Cell culture Biophysics Receptors Virus sense organs Poliovirus Receptor |
| Zdroj: | Virology. 197:501-505 |
| ISSN: | 0042-6822 |
| Popis: | Soluble extracts of Spodoptera frugiperda cells expressing the poliovirus receptor (PVR) induce the native poliovirus (PV) to "A" particle conformational change (J. Virol. 64, 4697-4702). We describe the variables that regulate this passage and study the suitability of solubilized PVR both for use as an in vitro system to characterize the receptor-mediated conformational alteration and for the production of large amounts of altered virus for structural analysis. PVR seems to function in a stoichiometric fashion and the A particles produced appear as intact, stain excluding, spherical structures by electron microscopy, regardless of the extensive proteolysis of the capsid protein VP1, which takes place during the conversion. The products obtained, time course, and temperature and ionic strength dependence of the alteration of PV by the solubilized PVR are indistinguishable from those of the alteration that leads to productive infection in cultured cells. Therefore, solubilized PVR may provide a convenient in vitro system for further characterization of the cell entry process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|