μ-Opioid receptor inhibition of substance P release from primary afferents disappears in neuropathic pain but not inflammatory pain
| Autor: | Wenling Chen, James A. McRoberts, Juan Carlos G. Marvizón |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Receptors Opioid mu Pain Substance P Article Rats Sprague-Dawley Sciatica chemistry.chemical_compound Dorsal root ganglion Ganglia Spinal Internal medicine Tachykinin receptor 1 medicine Animals Inflammation Neurons Dose-Response Relationship Drug General Neuroscience Enkephalin Ala(2)-MePhe(4)-Gly(5) Receptors Neurokinin-1 Nerve injury Sciatic Nerve nervous system diseases Rats Analgesics Opioid Disease Models Animal DAMGO medicine.anatomical_structure Endocrinology Nociception Spinal Cord chemistry Hyperalgesia Anesthesia Neuropathic pain Sciatic nerve medicine.symptom psychological phenomena and processes |
| Zdroj: | Neuroscience. 267:67-82 |
| ISSN: | 0306-4522 |
| DOI: | 10.1016/j.neuroscience.2014.02.023 |
| Popis: | Opiate analgesia in the spinal cord is impaired during neuropathic pain. We hypothesized that this is caused by a decrease in μ-opioid receptor inhibition of neurotransmitter release from primary afferents. To investigate this possibility, we measured substance P release in the spinal dorsal horn as neurokinin 1 receptor (NK1R) internalization in rats with chronic constriction injury (CCI) of the sciatic nerve. Noxious stimulation of the paw with CCI produced inconsistent NK1R internalization, suggesting that transmission of nociceptive signals by the injured nerve was variably impaired after CCI. This idea was supported by the fact that CCI produced only small changes in the ability of exogenous substance P to induce NK1R internalization or in the release of substance P evoked centrally from site of nerve injury. In subsequent experiments, NK1R internalization was induced in spinal cord slices by stimulating the dorsal root ipsilateral to CCI. We observed a complete loss of the inhibition of substance P release by the μ-opioid receptor agonist [D-Ala(2), NMe-Phe(4), Gly-ol(5)]-enkephalin (DAMGO) in CCI rats but not in sham-operated rats. In contrast, DAMGO still inhibited substance P release after inflammation of the hind paw with complete Freund's adjuvant and in naïve rats. This loss of inhibition was not due to μ-opioid receptor downregulation in primary afferents, because their colocalization with substance P was unchanged, both in dorsal root ganglion neurons and primary afferent fibers in the dorsal horn. In conclusion, nerve injury eliminates the inhibition of substance P release by μ-opioid receptors, probably by hindering their signaling mechanisms. |
| Databáze: | OpenAIRE |
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