Acoustofluidic-mediated molecular delivery to human T cells with a three-dimensional-printed flow chamber
| Autor: | Connor S. Centner, John T. Moore, Mary E. Baxter, Zachary T. Long, Jacob M. Miller, Ekaterina S. Kovatsenko, Benjamin Xie, Michael A. Menze, R. Eric Berson, Paula J. Bates, Kavitha Yaddanapudi, Jonathan A. Kopechek |
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| Rok vydání: | 2022 |
| Předmět: |
0303 health sciences
03 medical and health sciences Drug Delivery Systems Microbubbles Acoustics and Ultrasonics Arts and Humanities (miscellaneous) T-Lymphocytes Humans 02 engineering and technology 021001 nanoscience & nanotechnology 0210 nano-technology 030304 developmental biology Ultrasonography |
| Zdroj: | The Journal of the Acoustical Society of America. 150(6) |
| ISSN: | 1520-8524 |
| Popis: | Cell-based therapies have garnered significant interest to treat cancer and other diseases. Acoustofluidic technologies are in development to improve cell therapy manufacturing by facilitating rapid molecular delivery across the plasma membrane via ultrasound and microbubbles (MBs). In this study, a three-dimensional (3D) printed acoustofluidic device was used to deliver a fluorescent molecule, calcein, to human T cells. Intracellular delivery of calcein was assessed after varying parameters such as MB face charge, MB concentration, flow channel geometry, ultrasound pressure, and delivery time point after ultrasound treatment. MBs with a cationic surface charge caused statistically significant increases in calcein delivery during acoustofluidic treatment compared to MBs with a neutral surface charge (p0.001). Calcein delivery was significantly higher with a concentric spiral channel geometry compared to a rectilinear channel geometry (p0.001). Additionally, calcein delivery was significantly enhanced at increased ultrasound pressures of 5.1 MPa compared to lower ultrasound pressures between 0-3.8 MPa (p0.001). These results demonstrate that a 3D-printed acoustofluidic device can significantly enhance intracellular delivery of biomolecules to T cells, which may be a viable approach to advance cell-based therapies. |
| Databáze: | OpenAIRE |
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