Restoration of the self-splicing activity of a defective group II intron by a small trans-acting RNA
| Autor: | Mathias Suchy, Carlo Schmelzer |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Genetics
Small RNA Spliceosome Base Sequence biology RNA Splicing DNA Mutational Analysis Molecular Sequence Data Ribozyme Intron RNA Hydrogen Bonding RNA Fungal Saccharomyces cerevisiae Group II intron Catalysis Introns Mitochondria Structure-Activity Relationship Exon Structural Biology RNA splicing biology.protein Molecular Biology |
| Zdroj: | Journal of Molecular Biology. 222:179-187 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/0022-2836(91)90204-j |
| Popis: | The yeast mitochondrial group II intron bI1 is self-splicing in vitro. We have introduced a deletion of hairpin C1 within the structural domain 1 that abolishes catalytic activity of the intron in the normal splicing reaction in cis, but does less severely affect a reaction in trans, the reopening of ligated exons. Since exon reopening is supposed to correspond to a reverse 3' cleavage this suggests that the deletion specifically blocks the first reaction step. The intron regains its activity to self-splice in cis by intermolecular complementation with a small RNA harbouring sequences lacking in the mutant intron. These results demonstrate the feasibility to reconstitute a functionally active structure of the truncated intron by intermolecular complementation in vitro. Furthermore, the data support the hypothesis that group II introns are predecessors of nuclear pre-mRNA introns and that the small nuclear RNAs of the spliceosome arose by segregation from the original intron. |
| Databáze: | OpenAIRE |
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