Imaging and Analysis of Mitochondrial Dynamics in Living Cells
| Autor: | Ekanayake, Sanjaya, Zawily, Amr, Paszkiewicz, Gaël, Rolland, Aurélia, LOGAN, David, Whelan, James, Murcha, Monica |
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| Přispěvatelé: | University of Saskatchewan - Department of Biology, University of Saskatchewan [Saskatoon] (U of S), Damanhour University [Egypte], Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), La Trobe University, La Trobe University [Melbourne], Centre of Excellence in Plant Energy Biology (ARC), Australian National University (ANU)-School of Biochemistry and Molecular Biology, Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA) |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0303 health sciences [SDV]Life Sciences [q-bio] Dynamics (mechanics) Mutant Motility Mitochondrion Biology 01 natural sciences Cytoplasmic streaming Cell biology 03 medical and health sciences Organelle Fluorescent protein Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493926381 Plant Mitochondria: methods and protocol Plant Mitochondria: methods and protocol, 1305, Humana Press, pp.223-40, 2015, Methods in Molecular Biology ; 1305, 978-1-4939-2639-8. ⟨10.1007/978-1-4939-2639-8_16⟩ |
| DOI: | 10.1007/978-1-4939-2639-8_16 |
| Popis: | One of the most striking features of plant mitochondria when visualized in living tissue is their dynamism. The beauty of cytoplasmic streaming, driving, and being driven by the motility of mitochondria and other small organelles belies the complexity of the process. Equally, capturing that dynamism and investigating the genes, proteins, and mechanisms underpinning the processes using molecular cell biology and bioimaging is a complex process. It requires the generation of fluorescent protein constructs, stable transgenic plants sometimes expressing multiple fusions, and generation of mutants, even before one is ready for analytical experimentation. Here, we describe some of the key tools and methods necessary to investigate plant mitochondrial dynamics. |
| Databáze: | OpenAIRE |
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