HPLC Quantitative Analysis of Rhein and Antidermatophytic Activity of Cassia fistula Pod Pulp Extracts of Various Storage Conditions
| Autor: | Savita Chewchinda, Mansuang Wuthi-udomlert, Wandee Gritsanapan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Antifungal Agents
Article Subject Preservation Biological Cassia lcsh:Medicine Decoction Anthraquinones engineering.material Anthraquinone High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Botany Enzyme Inhibitors Chromatography High Pressure Liquid Chromatography General Immunology and Microbiology biology Plant Extracts Pulp (paper) Broth microdilution lcsh:R General Medicine biology.organism_classification Point of delivery chemistry engineering Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2013 (2013) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2013/821295 |
| Popis: | Cassia fistulais well known for its laxative and antifungal properties due to anthraquinone compounds in the pods. This study quantitatively analyzed rhein in theC. fistulapod pulp decoction extracts kept under various storage conditions using HPLC. The antifungal activity of the extracts and their hydrolyzed mixture was also evaluated against dermatophytes. The contents of rhein in all stored decoction extracts remained more than 95% (95.69–100.66%) of the initial amount (0.0823±0.001% w/w). There was no significant change of the extracts kept in glass vials and in aluminum foil bags. The decoction extract ofC. fistulapod pulp and its hydrolyzed mixture containing anthraquinone aglycones were tested against clinical strains of dermatophytes by broth microdilution technique. The results revealed good chemical and antifungal stabilities against dermatophytes ofC. fistulapod pulp decoction extracts stored under various accelerated and real time storage conditions. |
| Databáze: | OpenAIRE |
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