Video laryngoscopic oral intubation in rats: a simple and effective method
| Autor: | William J Cleveland, Tulasi Jinka, Matthias L. Riess, Claudius Balzer |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Male Physiology medicine.medical_treatment Endotracheal intubation Video-Assisted Surgery Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Otoscopes Physiology (medical) medicine Intubation Intratracheal Intubation Seldinger technique Animals Humans Mechanical ventilation Laryngoscopy business.industry Rat Larynx Cell Biology Respiration Artificial Stylet Rats Trachea 030104 developmental biology 030228 respiratory system Video laryngoscopy Anesthesia Innovative Methodology Larynx business Airway |
| Zdroj: | Am J Physiol Lung Cell Mol Physiol |
| Popis: | Endotracheal intubation is a vital component of many rat in vivo experiments to secure the airway and allow controlled ventilation. Even in the hands of experienced researchers, however, the procedure remains technically challenging. The safest and most reliable way for human intubation is by video laryngoscopy. Previous attempts to apply this technique in rodents have been complicated and expensive. We, hereby, describe a novel, noninvasive method to safely intubate rats orally by video laryngoscopy, thus avoiding the need for a surgical tracheostomy. By repurposing a commercially available ear wax removal device, visualization of the rat larynx can be significantly enhanced. Because of its small diameter, integrated illumination, and a powerful camera with adequate focal length, the device has all of the necessary properties for exploring the upper airway of a rat. After identifying the vocal cords by video laryngoscopy, the insertion of an endotracheal tube (a 14G intravenous catheter) into the trachea under constant visual control is facilitated by using PE50 polyethylene tubing as a stylet (Seldinger technique). The procedure has been performed more than 60 times in our laboratory; all intubations were successful on the first attempt, and no adverse events were observed. We conclude that the described procedure is a simple and effective way to intubate a rat noninvasively, using inexpensive and commercially available equipment. |
| Databáze: | OpenAIRE |
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