Respiratory syncytial virus infection induces a subset of types I and III interferons in human dendritic cells
| Autor: | Peter L. Collins, Cindy Luongo, Ursula J. Buchholz, Ronald G. Jubin, Maria B. dos Santos, Cyril Le Nouën, Lynnsie M. Schramm, Philippa Hillyer, Lei Huang, Ronald L. Rabin, Lihan Yan, Aaron Chen, Viraj P. Mane, Rachel Shepard |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Bronchi Respiratory Mucosa Respiratory Syncytial Virus Infections Biology Virus Article 03 medical and health sciences 0302 clinical medicine Interferon Virology medicine Humans Respiratory system Gene Lung Cells Cultured Inflammation Innate immune system Strain (chemistry) Interferon-stimulated gene virus diseases Interferon-alpha Epithelial Cells Dendritic cell Dendritic Cells Interferon-beta 030104 developmental biology Respiratory Syncytial Virus Human Immunology Cytokines 030215 immunology medicine.drug |
| Zdroj: | Virology. 504 |
| ISSN: | 1096-0341 |
| Popis: | Whether respiratory syncytial virus (RSV) induces severe infantile pulmonary disease may depend on viral strain and expression of types I and III interferons (IFNs). These IFNs impact disease severity by inducing expression of many anti-viral IFN-stimulated genes (ISGs). To investigate the impact of RSV strain on IFN and ISG expression, we stimulated human monocyte-derived DCs (MDDCs) with either RSV A2 or Line 19 and measured expression of types I and III IFNs and ISGs. At 24h, A2 elicited higher ISG expression than Line 19. Both strains induced MDDCs to express genes for IFN-β, IFN-α1, IFN-α8, and IFN-λ1-3, but only A2 induced IFN-α2, -α14 and -α21. We then show that IFN-α8 and IFN-α14 most potently induced MDDCs and bronchial epithelial cells (BECs) to express ISGs. Our findings demonstrate that RSV strain may impact patterns of types I and III IFN expression and the magnitude of the ISG response by DCs and BECs. |
| Databáze: | OpenAIRE |
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