Acute changes in temperature or oxygen availability induce ROS fluctuations in Daphnia magna linked with fluctuations of reduced and oxidized glutathione, catalase activity and gene (haemoglobin) expression
| Autor: | Dörthe Becker, Bettina Zeis, Rüdiger J. Paul, Benjamin F. Brinkmann |
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| Rok vydání: | 2011 |
| Předmět: |
Mitochondrial ROS
Antioxidant medicine.medical_treatment Gene Expression Environment Redox Antioxidants chemistry.chemical_compound Hemoglobins Gene expression medicine Animals skin and connective tissue diseases Hypoxia Hyperoxia chemistry.chemical_classification Reactive oxygen species biology Glutathione Disulfide Temperature Cell Biology General Medicine Glutathione Catalase Oxidants Adaptation Physiological Cell biology Oxygen Biochemistry chemistry Daphnia biology.protein sense organs Hypoxia-Inducible Factor 1 medicine.symptom Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | Biology of the cell. 103(8) |
| ISSN: | 1768-322X |
| Popis: | Background information. ROS (reactive oxygen species) as well as components of the antioxidant redox systems may act as signals. To link acute environmental change with gene expression, changes in ROS and GSH/GSSG (reduced/oxidized glutathione) level were measured upon acute changes in temperature or oxygen availability in the aquatic key species Daphnia magna together with HIF-1 (hypoxia-inducible factor 1)-mediated Hb (haemoglobin) expression. Results. Acute exposures to 30°C or hypoxia, which induced tissue hypoxia (and possibly elevated mitochondrial ROS production), caused resembling fluctuations of ROS and GSH levels, with frequency and number of peaks increasing and their delay decreasing with the magnitude of environmental change (size of tissue hypoxia). Acute hyperoxia induced an initial decrease in ROS level. Evidence is also provided for the promoting effects of ROS on catalase activity. A signalling function of the ROS fluctuations upon acute changes in temperature was found in the case of Hb, the expression of which is known to respond to temperature changes, by detecting corresponding time courses of both transcription and protein formation. Conclusion. ROS-dependent signalling was affected by changes in temperature or oxygen availability. Feedback interactions between ROS and the glutathione redox system, possibly driven by elevated mitochondrial ROS production, likely contributed to the appearance of the ROS and GSH fluctuations upon acute environmental change. Fluctuating ROS levels, which reflect for the magnitude of environmental change, could be a way to transfer information on ROS production to subsequent processes (gene expression) while avoiding too-high and damaging ROS levels. |
| Databáze: | OpenAIRE |
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