Standardisation of flow cytometry for whole blood immunophenotyping of islet transplant and transplant clinical trial recipients

Autor:	Yi Vee Chew, Xin Maggie Wang, Shounan Yi, Lindy Williams, Stephen I. Alexander, Karen Keung, Heather Burns, Patricia Anderson, Wayne J. Hawthorne, Negar Talaei Zanjani, Leigh Nicholson, Min Hu, Elvira Jimenez Vera, Ellis Patrick, Hsiao-Ting Chen, Philip J. O'Connell, Suat Dervish
Rok vydání:	2019
Předmět:	B Cells Cell Transplantation Islets of Langerhans Transplantation Monocytes White Blood Cells 0302 clinical medicine Immunophenotyping Animal Cells Medicine and Health Sciences Lymphocytes Whole blood 0303 health sciences Clinical Trials as Topic Multidisciplinary medicine.diagnostic_test biology T Cells Islet Transplantation Regulatory T cells Flow Cytometry 3. Good health Specimen collection Medicine Sample collection Antibody Cellular Types Research Article Endocrine System Procedures Science Immune Cells Immunology Cytotoxic T cells Surgical and Invasive Medical Procedures Antibodies Flow cytometry 03 medical and health sciences Antigen medicine Humans Antibody-Producing Cells 030304 developmental biology Transplantation Blood Cells business.industry Biology and Life Sciences Cell Biology Transplant Recipients Diabetes Mellitus Type 1 Case-Control Studies biology.protein business 030215 immunology Granulocytes
Zdroj:	PLoS ONE PLoS ONE, Vol 14, Iss 5, p e0217163 (2019)
ISSN:	1932-6203
Popis:	Understanding the immunological phenotype of transplant recipients is important to improve outcomes and develop new therapies. Immunophenotyping of whole peripheral blood (WPB) by flow cytometry is a rapid method to obtain large amounts of data relating to the outcomes of different transplant treatments with limited patient impact. Healthy individuals and patients with type 1 diabetes (T1D) enrolled in islet transplantation were recruited and WPB was collected. 46 fluorochrome-conjugated mouse-anti-human antibodies were used (43 of 46 antibodies were titrated). BD cytometer setup and tracking beads were used to characterize and adjust for cytometer performance. Antibody cocktails were pre-mixed
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3e8bff75112b7e526bc3b577883a259 https://pubmed.ncbi.nlm.nih.gov/31116766 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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