A novel method for the quality control of saffron through the simultaneous analysis of authenticity and adulteration markers by liquid chromatography-(quadrupole-time of flight)-mass spectrometry
| Autor: | María Luisa Marina, Antonio L. Crego, María Castro-Puyana, Miguel Guijarro-Díez |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Quality Control
Formic acid Mass spectrometry 01 natural sciences Mass Spectrometry Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Liquid chromatography–mass spectrometry Chromatography High Pressure Liquid Crocus Adulterant Chromatography biology Elution 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences chemistry Gardenia Kaempferol Chromatography Liquid Food Science |
| Zdroj: | Food Chemistry. 228:403-410 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2017.02.015 |
| Popis: | A liquid chromatography-(quadrupole-time of flight)-mass spectrometry methodology was developed to assess the authenticity of saffron through the analysis of a group of kaempferol derivatives recently proposed as novel authenticity markers as a result of a metabolomic study of saffron (kaempferol 3-O-glucoside, kaempferol 3-O-sophoroside, kaempferol 3,7-O-diglucoside, kaempferol 3,7,4'-O-triglucoside, kaempferol 3-O-sophoroside-7-O-glucoside). Geniposide was also studied as an adulteration marker of saffron with gardenia. The optimized chromatographic conditions enabling the simultaneous separation of glycosylated kaempferols and geniposide consisted of the use of a C18 column and an elution gradient with acetonitrile and water as mobile phases (both with formic acid at 0.1%). A strategy was proposed to evaluate the minimum quantifiable adulteration percentage which was established at a 0.2% regardless of the adulterant employed. The analysis of nineteen commercial samples showed the method to be specific and suitable for saffron quality control. |
| Databáze: | OpenAIRE |
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