Evaluation of the Range of Clinical Presentations of Herpes Simplex Encephalitis by Using Polymerase Chain Reaction Assay of Cerebrospinal Fluid Samples
| Autor: | Fred D. Lakeman, Ana Maria Crous Tsanaclis, Matthew S. Mayo, Cláudio Sérgio Pannuti, Renan Barros Domingues |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Microbiology (medical) Pathology medicine.medical_specialty Tomography Scanners X-Ray Computed Adolescent medicine.disease_cause Polymerase Chain Reaction Herpesviridae Virus Cerebrospinal fluid Alphaherpesvirinae Biopsy medicine Humans Encephalitis Viral Prospective Studies Child Aged Aged 80 and over medicine.diagnostic_test biology business.industry Brain biopsy Infant Electroencephalography Herpes Simplex Middle Aged medicine.disease biology.organism_classification Magnetic Resonance Imaging Virology Infectious Diseases Herpes simplex virus Child Preschool DNA Viral business Encephalitis |
| Zdroj: | Clinical Infectious Diseases. 25:86-91 |
| ISSN: | 1537-6591 1058-4838 |
| Popis: | Detection of DNA from herpes simplex virus in cerebrospinal fluid (CSF) samples by polymerase chain reaction (PCR) analysis has been shown to be more sensitive and specific for the diagnosis of herpes simplex encephalitis than isolation of herpes simplex virus from biopsy specimens of brain tissue. Because of the invasiveness of brain biopsy, it has been suggested that PCR analysis of CSF may reveal a wider spectrum of the disease than has been previously recognized by brain biopsy studies. In this study, PCR assay of CSF samples obtained from 29, 12, and 8 patients with focal, mild, and diffuse encephalitis, respectively, was performed. PCR assay was positive for 15 (51.7%) of 29 patients with focal encephalitis and three (25%) of 12 patients with mild encephalitis. The correlation between temporal abnormalities shown by electroencephalography, computed tomography of the brain, or cranial magnetic resonance imaging and a positive PCR assay was high. PCR analysis has revealed that atypical and less severe forms of encephalitis are caused by herpes simplex virus. |
| Databáze: | OpenAIRE |
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