Use of Encapsulated Single Chain Antibodies for Induction of Anti-Idiotypic Humoral and Cellular Immune Responses
| Autor: | Jie Ma, Antoine A. Noujaim, John Samuel, Ragupathy Madiyalakan, Glen S. Kwon, Dong Luo |
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| Rok vydání: | 1998 |
| Předmět: |
endocrine system diseases
Polymers medicine.medical_treatment Pharmaceutical Science chemical and pharmacologic phenomena Cancer Vaccines Epitope Mice chemistry.chemical_compound Drug Delivery Systems Immune system Polylactic Acid-Polyglycolic Acid Copolymer Antigen medicine Animals Humans Lactic Acid Drug Carriers Mice Inbred BALB C biology Immunotherapy Microspheres female genital diseases and pregnancy complications Antibodies Anti-Idiotypic PLGA chemistry CA-125 Antigen Immunology biology.protein Female Cancer vaccine Antibody Polyglycolic Acid Single-Chain Antibodies |
| Zdroj: | Journal of Pharmaceutical Sciences. 87:1375-1378 |
| ISSN: | 0022-3549 |
| DOI: | 10.1021/js9800681 |
| Popis: | The use of biodegradable poly(lactic-co-glycolic acid) (PLGA) microspheres as a cancer vaccine delivery system for induction of anti-idiotypic responses has been investigated using a single chain antibody scFv-pDL10, which recognizes the human ovarian cancer antigen CA125. Immunization of mice with scFv-pDL10 encapsulated in PLGA microspheres resulted in enhanced humoral and cellular immune responses when compared to scFv-pDL10 alone. Induced anti-idiotypic antibodies (Ab2) which mimic the original antigen CA125 compete with CA125 for the epitope. A cellular response (T2 induction) was also observed. These results raise the possibility of anti-idiotypic antibody induction by a single chain antibody, encapsulated in biodegradeble microspheres, as a potential vaccine for ovarian carcinoma. |
| Databáze: | OpenAIRE |
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