Effect of a 2-HP-β-Cyclodextrin Formulation on the Biological Transport and Delivery of Chemotherapeutic PLGA Nanoparticles
| Autor: | Zeju Huang, Haosen Luo, Kangyu Zheng, Jiaying Huang, Yanzhong Chen, Xin Huang, Junfeng Ban, Zhufen Lu, Xiangmei Liu, Qingchun Xie, Zhicong Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Lung Neoplasms Pharmaceutical Science Nanoparticle Rats Sprague-Dawley chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Polylactic Acid-Polyglycolic Acid Copolymer Drug Discovery Tissue Distribution sustained release Cytotoxicity pH-responsive Original Research chemistry.chemical_classification Drug Carriers Cyclodextrin 2-Hydroxypropyl-beta-cyclodextrin PLGA Paclitaxel 030220 oncology & carcinogenesis Area Under Curve Emulsion Female Biodistribution 2-hydroxypropyl-β-cyclodextrin macromolecular substances cancer treatment 03 medical and health sciences In vivo Animals Humans Particle Size Pharmacology Drug Design Development and Therapy technology industry and agriculture long-circulating Biological Transport PLGA nanoparticle Antineoplastic Agents Phytogenic Rats Drug Liberation 030104 developmental biology chemistry A549 Cells Biophysics Nanoparticles |
| Zdroj: | Drug Design, Development and Therapy |
| ISSN: | 1177-8881 |
| Popis: | Kangyu Zheng,1,2,* Zeju Huang,1,2 Jiaying Huang,1,2 Xiangmei Liu,3,* Junfeng Ban,1,2,* Xin Huang,1,2 Haosen Luo,1,2 Zhicong Chen,1,2 Qingchun Xie,1,2 Yanzhong Chen,1,2 Zhufen Lu1,2 1Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, People‘s Republic of China; 2Guangdong Provincial Engineering Center of Topical Precision Drug Delivery System, Center for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, People‘s Republic of China; 3Guangzhou Quality Supervision and Testing Institute, Guangzhou, People‘s Republic of China*These authors contributed equally to this workCorrespondence: Yanzhong Chen; Zhufen LuGuangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, 510006, People’s Republic of ChinaTel +86 20 39352506Email doctor.c@163.com; luzhufen@163.comBackground: The aim of this work was to develop a novel and feasible modification strategy by utilizing the supramolecular effect of 2-hydroxypropyl-beta-cyclodextrin (2-HP-β-CD) for enhancing the biological transport efficiency of paclitaxel (PTX)-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles.Methods: PTX-loaded 2-HP-β-CD-modified PLGA nanoparticles (2-HP-β-CD/PLGA NPs) were prepared using the modified emulsion method. Nano-characteristics, drug release behavior, in vitro cytotoxicity, cellular uptake profiles and in vivo bio-behavior of the nanoparticles were then characterized.Results: Compared with the plain PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited smaller particle sizes (151.03± 1.36 nm), increased entrapment efficiency (∼ 49.12% increase) and sustained drug release. When added to A549 human lung cancer cells, compared with PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited higher cytotoxicity in MTT assays and improved cellular uptake efficiency. Pharmacokinetic analysis showed that the AUC value of 2-HP-β-CD/PLGA NPs was 2.4-fold higher than commercial Taxol® and 1.7-fold higher than plain PLGA NPs. In biodistribution assays, 2-HP-β-CD/PLGA NPs exhibited excellent stability in the circulation.Conclusion: The results of this study suggest that the formulation that contains 2-HP-β-CD can prolong PTX release, enhance drug transport efficiency and serve as a potential tumor targeting system for PTX.Keywords: PLGA nanoparticle, 2-hydroxypropyl-β-cyclodextrin, sustained release, pH-responsive, long-circulating, cancer treatment |
| Databáze: | OpenAIRE |
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