Metagenomics Analysis to Investigate the Microbial Communities and Their Functional Profile During Cyanobacterial Blooms in Lake Varese

Autor: Andrea Beghi, Pietro Genoni, Chiara Facca, Teresa Lettieri, Diana C. António, Franca Pandolfi, Patrizia Pretto, Robert Loos, Helle Skejo, Isabella Sanseverino, Armin Lahm
Rok vydání: 2021
Předmět:
Zdroj: Microbial Ecology. 83:850-868
ISSN: 1432-184X
0095-3628
DOI: 10.1007/s00248-021-01914-5
Popis: Toxic cyanobacterial blooms represent a natural phenomenon caused by a mass proliferation of photosynthetic prokaryotic microorganisms in water environments. Bloom events have been increasingly reported worldwide and their occurrence can pose serious threats to aquatic organisms and human health. In this study, we assessed the microbial composition, with a focus on Cyanobacteria, in Lake Varese, a eutrophic lake located in northern Italy. Water samples were collected and used for obtaining a 16S-based taxonomic profile and performing a shotgun sequencing analysis. The phyla found to exhibit the greatest relative abundance in the lake included Proteobacteria, Cyanobacteria, Actinobacteriota and Bacteroidota. In the epilimnion and at 2.5 × Secchi depth, Cyanobacteria were found to be more abundant compared to the low levels detected at greater depths. The blooms appear to be dominated mainly by the species Lyngbya robusta, and a specific functional profile was identified, suggesting that distinct metabolic processes characterized the bacterial population along the water column. Finally, analysis of the shotgun data also indicated the presence of a large and diverse phage population.
Databáze: OpenAIRE
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