Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance
| Autor: | M. Raghurami Reddy, Satendra K. Mangrauthia, M. Sheshu Madhav, Ramakrishna Chopperla, V. Venkatesh, D. Ladhalakshmi, E. Punniakotti, Raman Meenakshi Sundaram, Talluri Bhaskar Rao, Ramesh Methre, Gouri Sankar Laha, Sena M. Balachandran, B. Sailaja, A. Yugander |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Genes Plant 01 natural sciences Microbiology Rhizoctonia Rhizoctonia solani Transcriptome 03 medical and health sciences Transformation Genetic RNA interference Gene Expression Regulation Plant Genetics Gene silencing RNA Messenger Pectinase Pathogen Disease Resistance Plant Diseases Oryza sativa biology Sequence Analysis RNA fungi food and beverages Oryza General Medicine equipment and supplies biology.organism_classification Plant disease 030104 developmental biology Polygalacturonase Disease Progression Pectins RNA Interference Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Plant molecular biology. 100(1-2) |
| ISSN: | 1573-5028 |
| Popis: | RNAi mediated silencing of pectin degrading enzyme of R. solani gives a high level of resistance against sheath blight disease of rice. Rice sheath blight disease caused by Rhizoctonia solani Kuhn (telemorph; Thanatephorus cucumeris) is one of the most devastating fungal diseases which cause severe loss to rice grain production. In the absence of resistant cultivars, the disease is currently managed through fungicides which add to environmental pollution. To explore the potential of utilizing RNA interference (RNAi)-mediated resistance against sheath blight disease, we identified genes encoding proteins and enzymes involved in the RNAi pathway in this fungal pathogen. The RNAi target genes were deciphered by RNAseq analysis of a highly virulent strain of the R. solani grown in pectin medium. Additionally, pectin metabolism associated genes of R. solani were analyzed through transcriptome sequencing of infected rice tissues obtained from six diverse rice cultivars. One of the key candidate gene AG1IA_04727 encoding polygalacturonase (PG), which was observed to be significantly upregulated during infection, was targeted through RNAi to develop disease resistance. Stable expression of PG-RNAi construct in rice showed efficient silencing of AG1IA_04727 and suppression of sheath blight disease. This study highlights important information about the existence of RNAi machinery and key genes of R. solani which can be targeted through RNAi to develop pathogen-derived resistance, thus opening an alternative strategy for developing sheath blight-resistant rice cultivars. |
| Databáze: | OpenAIRE |
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