An autophagy-independent role for LC3 in equine arteritis virus replication
| Autor: | Iryna Monastyrska, Peter J. M. Rottier, Fulvio Reggiori, Mustafa Ulasli, Cornelis A. M. de Haan, Jun-Lin Guan |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
autophagy
Diergeneeskunde Viral Proteins/metabolism Green Fluorescent Proteins Virus Replication/physiology Virus Replication medicine.disease_cause Virus RNA Transport Cell Line Arterivirus Geneeskunde Viral Proteins Mice Equartevirus/physiology Equartevirus arterivirus medicine LC3 Autophagy Animals Arterivirus Infections/metabolism Transport Vesicles Molecular Biology RNA Double-Stranded Coronavirus Green Fluorescent Proteins/metabolism Arterivirus Infections biology Cell Membrane/metabolism Cell Membrane Membrane Proteins RNA RNA virus Cell Biology biology.organism_classification Virology Basic Research Paper Viral replication Cell culture Microtubule-Associated Proteins/metabolism Transport Vesicles/metabolism Membrane Proteins/metabolism RNA Double-Stranded/metabolism double-membrane vesicles Microtubule-Associated Proteins nidoviruses |
| Zdroj: | Autophagy Monastyrska, I, Ulasli, M, Rottier, P J M, Guan, J-L, Reggiori, F & de Haan, C A M 2013, ' An autophagy-independent role for LC3 in equine arteritis virus replication ', Autophagy, vol. 9, no. 2, pp. 164-74 . https://doi.org/10.4161/auto.22743 Autophagy, 9(2), 1. Landes Bioscience |
| ISSN: | 1554-8635 1554-8627 |
| DOI: | 10.4161/auto.22743 |
| Popis: | Equine arteritis virus (EAV) is an enveloped, positive-strand RNA virus. Genome replication of EAV has been associated with modified intracellular membranes that are shaped into double-membrane vesicles (DMVs). We showed by immuno-electron microscopy that the DMVs induced in EAV-infected cells contain double-strand (ds)RNA molecules, presumed RNA replication intermediates, and are decorated with the autophagy marker protein microtubule-associated protein 1 light chain 3 (LC3). Replication of EAV, however, was not affected in autophagy-deficient cells lacking autophagy-related protein 7 (ATG7). Nevertheless, colocalization of DMVs and LC3 was still observed in these knockout cells, which only contain the nonlipidated form of LC3. Although autophagy is not required, depletion of LC3 markedly reduced the replication of EAV. EAV replication could be fully restored in these cells by expression of a nonlipidated form of LC3. These findings demonstrate an autophagy-independent role for LC3 in EAV replication. Together with the observation that EAV-induced DMVs are also positive for ER degradation-enhancing α-mannosidase-like 1 (EDEM1), our data suggested that this virus, similarly to the distantly-related mouse hepatitis coronavirus, hijacks the ER-derived membranes of EDEMosomes to ensure its efficient replication. |
| Databáze: | OpenAIRE |
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