Transient Loss of Voltage Control of Ca2+ Release in the Presence of Maurocalcine in Skeletal Muscle
| Autor: | László Csernoch, Michel De Waard, Michel Ronjat, Vincent Jacquemond, Sandrine Pouvreau, Bruno Allard, Jean-Marc Sabatier |
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| Přispěvatelé: | Physiologie intégrative, cellulaire et moléculaire (PICM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Physiology, Medical and Health Science Center, University of Debrecen Egyetem [Debrecen], ERT 62, Université de la Méditerranée - Aix-Marseille 2-Ambrilia Biopharma S.A., Canaux calciques , fonctions et pathologies, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), CNRS, University Claude Bernard Lyon 1, Association Française contre les Myopathies., Collaboration, Canepari, Marco, Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, University of Debrecen |
| Rok vydání: | 2006 |
| Předmět: |
Patch-Clamp Techniques
[SDV.MHEP.PHY] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] MESH: Muscle Contraction Muscle Fibers Skeletal Calcium in biology MESH: Ryanodine Receptor Calcium Release Channel Membrane Potentials Mice MESH: Scorpion Venoms 0302 clinical medicine MESH: Animals Elméleti orvostudományok Membrane potential MESH: Muscle Skeletal 0303 health sciences MESH: Muscle Fibers Skeletal Ryanodine receptor Orvostudományok musculoskeletal system Sarcoplasmic Reticulum medicine.anatomical_structure MESH: Calcium MESH: Sarcoplasmic Reticulum cardiovascular system Maurocalcine medicine.symptom Ion Channel Gating Muscle Contraction Muscle contraction medicine.medical_specialty Biophysics Scorpion Venoms chemistry.chemical_element [SDV.BC]Life Sciences [q-bio]/Cellular Biology In Vitro Techniques Calcium 03 medical and health sciences Internal medicine MESH: Patch-Clamp Techniques [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] medicine MESH: Membrane Potentials Animals Muscle Skeletal MESH: Mice [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology Membranes Cell Membrane Skeletal muscle Ryanodine Receptor Calcium Release Channel MESH: Ion Channel Gating Membrane repolarization Endocrinology chemistry 030217 neurology & neurosurgery MESH: Cell Membrane |
| Zdroj: | Biophysical Journal Biophysical Journal, 2006, 91 (6), pp.2206-2215. ⟨10.1529/biophysj.105.078089⟩ Biophysical Journal, Biophysical Society, 2006, 91 (6), pp.2206-2215. ⟨10.1529/biophysj.105.078089⟩ |
| ISSN: | 0006-3495 1542-0086 |
| Popis: | International audience; In skeletal muscle, sarcoplasmic reticulum (SR) calcium release is controlled by the plasma membrane voltage through interactions between the voltage-sensing dihydropyridine receptor (DHPr) and the ryanodine receptor (RYr) calcium release channel. Maurocalcine (MCa), a scorpion toxin peptide presenting some homology with a segment of a cytoplasmic loop of the DHPr, has been previously shown to strongly affect the activity of the isolated RYr. We injected MCa into mouse skeletal muscle fibers and measured intracellular calcium under voltage-clamp conditions. Voltage-activated calcium transients exhibited similar properties in control and in MCa-injected fibers during the depolarizing pulses, and the voltage dependence of calcium release was similar under the two conditions. However, MCa was responsible for a pronounced sustained phase of Ca(2+) elevation that proceeded for seconds following membrane repolarization, with no concurrent alteration of the membrane current. The magnitude of the underlying uncontrolled extra phase of Ca(2+) release correlated well with the peak calcium release during the pulse. Results suggest that MCa binds to RYr that open on membrane depolarization and that this interaction specifically alters the process of repolarization-induced closure of the channels. |
| Databáze: | OpenAIRE |
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