Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules

Autor:	Yoshikatsu Murooka, Yasuaki Nakanishi, Kouji Kasai, Makoto Fujie, Takashi Yamada, Shoji Usami
Rok vydání:	2000
Předmět:	Asparagine synthetase Nucleic acid sequence Bioengineering Nodule (medicine) Biology Molecular cloning Applied Microbiology and Biotechnology Biochemistry Complementary DNA Gene expression medicine Asparagine medicine.symptom Gene Biotechnology
Zdroj:	Journal of Bioscience and Bioengineering. 89:559-563
ISSN:	1389-1723
DOI:	10.1016/s1389-1723(00)80057-2
Popis:	Two cDNAs that encode asparagine synthetase were cloned from root nodules of Astragalus sinicus cv. Japan (Renge-sou). The expression of the transcripts was nodule-enhanced. The expression of both genes was reduced in nodules when (NH4)2SO4 was added to the culture medium. This is the first report of the inhibition of asparagine synthetase gene expression by ammonium in root nodules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3354a0927c2ef8c4dc348c64fea4374 https://doi.org/10.1016/s1389-1723(00)80057-2 Zobrazit plný text záznamu Full Text from ScienceDirect