New Aspects Towards a Molecular Understanding of the Allicin Immunostimulatory Mechanism via Colec12, MARCO, and SCARB1 Receptors
| Autor: | Ioana Roman, Vlad Al. Toma, Ana Maria Raluca Gherman, Eva Fischer-Fodor, Adrian Bogdan Tigu, Bogdan Sevastre, Marian Taulescu, Anca D. Farcaș, Marcel Pârvu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Anti-Inflammatory Agents
immunoglobulins Immunoglobulin secretion Antioxidants lcsh:Chemistry chemistry.chemical_compound Disulfides Lymphocytes Receptors Immunologic Receptor lcsh:QH301-705.5 Spectroscopy Receptors Scavenger biology Chemistry General Medicine Scavenger Receptors Class B Catalase Glutathione Computer Science Applications Anti-Bacterial Agents Molecular Docking Simulation Antigens CD19 mechanism Antineoplastic Agents allicin Catalysis CD19 Article Inorganic Chemistry Animals Humans Physical and Theoretical Chemistry Scavenger receptor Molecular Biology Allicin Macrophages scavenger receptors Organic Chemistry Sulfinic Acids Molecular biology In vitro SCARB1 Collectins Rats Oxidative Stress lcsh:Biology (General) lcsh:QD1-999 biology.protein Immunization |
| Zdroj: | International Journal of Molecular Sciences, Vol 20, Iss 15, p 3627 (2019) International Journal of Molecular Sciences Volume 20 Issue 15 |
| ISSN: | 1422-0067 |
| Popis: | The allicin pleiotropic effects, which include anti-inflammatory, anti-oxidant, anti-tumoral, and antibacterial actions, were well demonstrated and correlated with various molecular pathways. The immunostimulatory mechanism of allicin has not been elucidated however, there is a possible cytokine stimulation from immunoglobulin release caused by allicin. In this study, when Wistar female rats and CD19+ lymphocytes were treated with three different doses of allicin, immunoglobulins, glutathione, and oxidative stress markers were assayed. Molecular docking was performed between S-allylmercaptoglutathione (GSSA)&mdash a circulating form of allicin in in vivo systems formed by the allicin interaction with glutathione (GSH)&mdash and scavenger receptors class A and B from macrophages, as well as CD19+ B lymphocytes. Our data demonstrated a humoral immunostimulatory effect of allicin in rats and direct stimulation of B lymphocytes by S-allyl-mercapto-glutathione, both correlated with decreased catalase (CAT) activity. The molecular docking revealed that S-allyl-mercapto-glutathione interacting with Colec12, MARCO (class A), and SCARB1 (class B) scavenger receptors in in vitro tests demonstrates a direct stimulation of immunoglobulin secretion by GSSA in CD19+ B lymphocytes. These data collectively indicate that GSSA stimulates immunoglobulin secretion by binding on scavenger receptors class B type 1 (SCARB1) from CD19+ B lymphocytes. |
| Databáze: | OpenAIRE |
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