Isolation of miRNAs that target EGFR mRNA in human lung cancer
| Autor: | Tatsuo Ohira, Gaku Yamaguchi, Masakatsu Takanashi, Norihiko Ikeda, Masami Tanaka, Masahiko Kuroda, Koji Fujita |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Lung Neoplasms
Biophysics Biology medicine.disease_cause Biochemistry Downregulation and upregulation Carcinoma Non-Small-Cell Lung Cell Line Tumor microRNA medicine Carcinoma Humans RNA Messenger Epidermal growth factor receptor Lung cancer Molecular Biology Regulation of gene expression Cell Biology medicine.disease Up-Regulation respiratory tract diseases ErbB Receptors Gene Expression Regulation Neoplastic MicroRNAs Cell Transformation Neoplastic Cell culture Immunology Cancer research biology.protein Carcinogenesis HeLa Cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 420:411-416 |
| ISSN: | 0006-291X |
| Popis: | Lung cancer, predominantly non-small cell lung cancer (NSCLC), remains the leading cause of cancer-related deaths worldwide. Although epidermal growth factor receptor (EGFR) signaling is important and well studied with respect to NSCLC progression, little is known about how miRNAs mediate EGFR signaling to modulate tumorigenesis. To identify miRNAs that target EGFR, we performed a bioinformatics analysis and found that miR-542-5p down-regulates EGFR mRNA and protein expression in human lung cancer cells (H3255, A549, Hcc827). We observed increases in EGFR association with Ago2 in miR-542-5p-transfected cells. Interestingly, we observed an inverse correlation of miR-542-5p expression and EGFR protein levels in human lung cancer tissue samples, suggesting that miR-542-5p directly targets EGFR mRNA. Furthermore, we found that miR-542-5p inhibited the growth of human lung cancer cells. Our findings suggest that miR-542-5p may act as an important modulator of EGFR-mediated oncogenesis, with potential applications as a novel therapeutic target in lung cancer. |
| Databáze: | OpenAIRE |
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