Multimodal Measurements of Brain Tissue Metabolism and Perfusion in a Neonatal Model of Hypoxic-Ischaemic Injury
| Autor: | Mamadou Diop, Linshan Liu, Laura Morrison, Ilias Tachtsidis, Alan Bainbridge, Ajay Rajaram, Keith St. Lawrence, Matthew Kewin, Gemma Bale, Udunna C. Anazodo |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Swine Brain tissue broadband near-infrared spectroscopy 01 natural sciences 010309 optics cytochrome-c-oxidase 03 medical and health sciences Oxygen Consumption 0302 clinical medicine Internal medicine 0103 physical sciences medicine Animals Cytochrome c oxidase diffuse correlation spectroscopy Spectroscopy Near-Infrared medicine.diagnostic_test biology business.industry Chemistry multimodal Brain Magnetic resonance imaging Metabolism Diffuse correlation spectroscopy Pathophysiology Oxygen Perfusion Endocrinology Cerebral blood flow Positron emission tomography Cerebrovascular Circulation Hypoxia-Ischemia Brain biology.protein Cerebral tissue Nuclear medicine business 030217 neurology & neurosurgery |
| Zdroj: | Advances in Experimental Medicine and Biology ISBN: 9783030482367 Medical Biophysics Publications |
| Popis: | This is the first multimodal study of cerebral tissue metabolism and perfusion post-hypoxic-ischaemic (HI) brain injury using broadband near-infrared spectroscopy (bNIRS), diffuse correlation spectroscopy (DCS), positron emission tomography (PET) and magnetic resonance spectroscopy (MRS). In seven piglet preclinical models of neonatal HI, we measured cerebral tissue saturation (StO2), cerebral blood flow (CBF), cerebral oxygen metabolism (CMRO2), changes in the mitochondrial oxidation state of cytochrome c oxidase (oxCCO), cerebral glucose metabolism (CMRglc) and tissue biochemistry (Lac+Thr/tNAA). At baseline, the parameters measured in the piglets that experience HI (not controls) were 64 ± 6% StO2, 35 ± 11 ml/100 g/min CBF and 2.0 ± 0.4 μmol/100 g/min CMRO2. After HI, the parameters measured were 68 ± 6% StO2, 35 ± 6 ml/100 g/min CBF, 1.3 ± 0.1 μmol/100 g/min CMRO2, 0.4 ± 0.2 Lac+Thr/tNAA and 9.5 ± 2.0 CMRglc. This study demonstrates the capacity of a multimodal set-up to interrogate the pathophysiology of HIE using a combination of optical methods, MRS, and PET. |
| Databáze: | OpenAIRE |
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