Phenotypic Analysis of Mice Lacking the Highly Abundant Purkinje Cell- and Bipolar Neuron-Specific PCP2 Protein
| Autor: | Beverly H. Koller, MyTrang Nguyen, Amy R. Mohn, Rodney M. Feddersen |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Neurons
Ribosomal Proteins Retina Cerebellum Cell Purkinje cell Gene Expression Gene targeting Cell Biology Biology Mice Mutant Strains Cell biology Mice Purkinje Cells Cellular and Molecular Neuroscience Phenotype medicine.anatomical_structure Bipolar neuron Ultrastructure medicine Animals Molecular Biology Neuroscience Gene |
| Zdroj: | Molecular and Cellular Neuroscience. 9:63-76 |
| ISSN: | 1044-7431 |
| DOI: | 10.1006/mcne.1997.0606 |
| Popis: | ThePurkinje cell protein-2(Pcp2,also known as L7) gene is abundantly expressed only in Purkinje cells of the cerebellum and bipolar neurons of the retina. The spatiotemporal expression pattern of this gene suggests a role for PCP2 in Purkinje cell development or normal cell physiology. A PCP2-deficient mouse was created by gene targeting to test the hypothesis that it is required for Purkinje cell development or function. Although normally present in abundance, the absence of PCP2 in null animals caused no observable cerebellar abnormalities. Behavioral analysis reveals normal abilities for balance and coordination. Null cerebellum has normal Purkinje cell numbers, morphology, and ultrastructure. Retinal bipolar neurons appear similarly unaffected. Aged null animals (22 months) were also examined and no deficits were detected using the same behavioral and histologic analyses. Although the null animal does not reveal the function of PCP2, it does rule out an essential role for PCP2 in Purkinje cell development, in Purkinje cell survival, and in at least some aspects of cerebellar function. |
| Databáze: | OpenAIRE |
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