Effect of Cellular-Based Artificial Antigen Presenting Cells Expressing ICOSL, in T-cell Subtypes Differentiation and Activation
| Autor: | Tohid Kazemi, Ali Akbar Akbari, Hojjatollah Nozad Charoudeh, Mehdi Talebi, Behzad Baradaran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Artificial antigen presenting cell
T cell Population Pharmaceutical Science RM1-950 02 engineering and technology Biology 030226 pharmacology & pharmacy 03 medical and health sciences 0302 clinical medicine Artificial antigen presenting cells T-cell sub-types medicine Cytotoxic T cell IL-2 receptor General Pharmacology Toxicology and Pharmaceutics Interleukin-7 receptor education education.field_of_study FOXP3 021001 nanoscience & nanotechnology medicine.anatomical_structure Differentiation Cancer research ICOSL Therapeutics. Pharmacology 0210 nano-technology CD8 Research Article |
| Zdroj: | Advanced Pharmaceutical Bulletin Advanced Pharmaceutical Bulletin, Vol 11, Iss 3, Pp 537-542 (2021) |
| ISSN: | 2251-7308 2228-5881 |
| Popis: | Purposes: Effective and selective T-cell activation and proliferation during the T-cell expansion phase of a cellular adoptive immunotherapy method, challenging because recent studies revealed the importance of each subtype of T-cells in different immunologic strategies against tumors, like CAR-T cell therapies. Artificial antigen presenting cells (aAPCs) regarded as a natural way to manipulate T-cell subtypes activation and specific proliferation. In the current study, we utilized K562 cells based aAPC method expressing the ICOSL molecule, to evaluate T-cell subtypes differentiation rate and functional status. Methods: CD3+T-cells isolated and, co-cultured with ICOSL expressing K562 cells. After 4, 6, and 10 days selective CD markers of T-cell subtypes and each subtype's activity-related genes levels evaluated by qPCR methods. Results: During the culture period, CD4+ Th related phenotype reduced continuously, and in day 10th of culture CD4+ T-cell's population significantly reduced (P =0.029). In contrast, the CD8+ population ratio was ascending during the study period but was not statistically significant. FoxP3+CD25-, Treg population ratio was significantly increased during the time in comparison with the control group, as well as memory T-cell phenotypic marker, CD127+, expressing cells ratio. T-cell subpopulations activity-related genes expression levels evaluated too, and the Th1 related IL-2 and INF-γ reductions observed alongside regulatory T-cells gene (IL-10) and Cytotoxic T-cell's related gene (Geranzym-A) elevations. Conclusion: We concluded that the K562-ICOSL based aAPC system is working and effective in T-cell short to medium culture periods, and this approach preparing relatively selective milieu for CD8+ T-Cell differentiation and much less Treg differentiation. |
| Databáze: | OpenAIRE |
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