Chemical formation of hybrid di-nitrogen calls fungal codenitrification into question
| Autor: | Andrew M. S. McMillan, Gwen Grelet, Bevan S. Weir, Bongkeun Song, Rebecca L. Phillips, Craig R. Tobias, Thilak Palmada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromatography Gas Denitrification Nitrogen Nitric-oxide reductase Nitrous Oxide chemistry.chemical_element Biology Greenhouse Gases 03 medical and health sciences chemistry.chemical_compound Anaerobiosis Abiotic component Multidisciplinary Ecology Fungi 04 agricultural and veterinary sciences Nitrous oxide Corrigenda Anoxic waters Aerobiosis 030104 developmental biology chemistry Anammox Environmental chemistry Nitrosation 040103 agronomy & agriculture 0401 agriculture forestry and fisheries |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep39077 |
| Popis: | Removal of excess nitrogen (N) can best be achieved through denitrification processes that transform N in water and terrestrial ecosystems to di-nitrogen (N2) gas. The greenhouse gas nitrous oxide (N2O) is considered an intermediate or end-product in denitrification pathways. Both abiotic and biotic denitrification processes use a single N source to form N2O. However, N2 can be formed from two distinct N sources (known as hybrid N2) through biologically mediated processes of anammox and codenitrification. We questioned if hybrid N2 produced during fungal incubation at neutral pH could be attributed to abiotic nitrosation and if N2O was consumed during N2 formation. Experiments with gas chromatography indicated N2 was formed in the presence of live and dead fungi and in the absence of fungi, while N2O steadily increased. We used isotope pairing techniques and confirmed abiotic production of hybrid N2 under both anoxic and 20% O2 atmosphere conditions. Our findings question the assumptions that (1) N2O is an intermediate required for N2 formation, (2) production of N2 and N2O requires anaerobiosis, and (3) hybrid N2 is evidence of codenitrification and/or anammox. The N cycle framework should include abiotic production of N2. |
| Databáze: | OpenAIRE |
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