Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum
| Autor: | Jędrzej Pankowski, Paweł Jedynak, Jakub Lang, Piotr Grabarz, Bartosz Pluciński |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Photosystem II
Callus formation seed-derived callus Nicotiana tabacum Developmental research Germination 2 4-D General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound cytokinin Plant Growth Regulators callus Benzyl Compounds Tobacco Photosynthesis biology Indoleacetic Acids Chemistry Chlorophyll A Sowing Kinetin biology.organism_classification BAP Horticulture Purines Seedlings Callus Seeds 2 4-Dichlorophenoxyacetic Acid Mesophyll Cells auxin Hormone |
| Popis: | Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid – IAA; 2,4-Dichlorophenoxyacetic acid – 2,4-D; kinetin – KIN; 6-Benzylaminopurine – BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basis of fluorescence of chlorophyll a in photosystem II) were noticed. |
| Databáze: | OpenAIRE |
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