Superoxide dismutase in human sperm suspensions: Relationship with cellular composition, oxidative stress, and sperm function
| Autor: | D S Irvine, A Carreras, Robert John Aitken, D W Buckingham |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Cell Separation Glucosephosphate Dehydrogenase In Vitro Techniques Sensitivity and Specificity Biochemistry Cell Fusion Superoxide dismutase Cricetinae Physiology (medical) Centrifugation Density Gradient Leukocytes medicine Animals Humans Creatine Kinase Sperm motility Sperm-Ovum Interactions Sperm Count Spermatozoon biology Superoxide Dismutase urogenital system Povidone Silicon Dioxide Oocyte Spermatozoa Sperm Cell biology Oxidative Stress medicine.anatomical_structure Luminescent Measurements Sperm Motility biology.protein Female Lipid Peroxidation Acrosome Percoll Spermatogenesis |
| Zdroj: | Free Radical Biology and Medicine. 21:495-504 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/0891-5849(96)00119-0 |
| Popis: | Sensitive techniques have been developed for monitoring superoxide dismutase (SOD) activities in human sperm preparations. In contradiction to the protective role normally assigned to SOD, populations of defective spermatozoa recovered from the low density region of Percoll gradients were found to have three times more SOD than functionally competent preparations pelleting in high density Percoll. SOD activity was negatively correlated with the movement characteristics of human spermatozoa and their capacity for oocyte fusion, and positively associated with the induction of peroxidative damage. SOD activity was also highly correlated with other markers of the cytoplasmic space, creatine kinase (CK), and glucose-6-phosphate dehydrogenase (G-6-PDH). We conclude that while SOD may play a physiological role in maintaining a balance between O2.- and H2O2, high levels of this enzyme are associated with impaired sperm function because (a) the human spermatozoon is highly susceptible to the cytotoxic effects of H2O2, (b) O2.- is an important mediator of normal sperm function, and (c) high SOD activities reflect errors in spermatogenesis associated with germ cell exfoliation and the retention of excess residual cytoplasm by the spermatozoa. |
| Databáze: | OpenAIRE |
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