Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42
| Autor: | Sara M. El-Ewasy, Nancy M. El-Shweihy, Noura El-Ahmady El-Naggar |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Streptomyces cavourensis DNA Bacterial Cholesterol oxidase Cell Culture Techniques Microbiology Streptomyces 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins RNA Ribosomal 16S Extracellular Phylogeny Enzyme Assays Analysis of Variance biology Plackett–Burman design Cholesterol Cholesterol Oxidase Hydrogen-Ion Concentration biology.organism_classification Classification Culture Media Enzyme Activation 030104 developmental biology Biochemistry chemistry Cholesterol oxidase activity Ammonium Sulfate Genes Bacterial Fermentation Microscopy Electron Scanning Erratum Sequence Alignment |
| Zdroj: | BMC Microbiology |
| ISSN: | 1471-2180 |
| Popis: | Due to broad range of clinical and industrial applications of cholesterol oxidase, isolation and screening of bacterial strains producing extracellular form of cholesterol oxidase is of great importance. One hundred and thirty actinomycete isolates were screened for their cholesterol oxidase activity. Among them, a potential culture, strain NEAE-42 is displayed the highest extracellular cholesterol oxidase activity. It was selected and identified as Streptomyces cavourensis strain NEAE-42. The optimization of different process parameters for cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42 using Plackett–Burman experimental design and response surface methodology was carried out. Fifteen variables were screened using Plackett–Burman experimental design. Cholesterol, initial pH and (NH4)2SO4 were the most significant positive independent variables affecting cholesterol oxidase production. Central composite design was chosen to elucidate the optimal concentrations of the selected process variables on cholesterol oxidase production. It was found that, cholesterol oxidase production by Streptomyces cavourensis strain NEAE-42 after optimization process was 20.521U/mL which is higher than result obtained from the basal medium before screening process using Plackett-Burman (3.31 U/mL) with a fold of increase 6.19. The cholesterol oxidase level production obtained in this study (20.521U/mL) by the statistical method is higher than many of the reported values. |
| Databáze: | OpenAIRE |
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