Ectopic expression of a phytochrome B gene from Chinese cabbage (Brassica rapa L. ssp. pekinensis) in Arabidopsis thaliana promotes seedling de-etiolation, dwarfing in mature plants, and delayed flowering
| Autor: | Shu Zhang, Liang Su, Jing-Juan Li, Jianwei Gao, Lin Guo, Meifang Song, Hong-Zhong Shang, Jian-Ping Yang, Yang Xiao, Hou Pei, Hai-Ke Gu |
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| Rok vydání: | 2015 |
| Předmět: |
Time Factors
Light Molecular Sequence Data Arabidopsis Gene Expression Flowers Plant Science Biology Plant Growth Regulators Gene Expression Regulation Plant Phytochrome B Etiolation Brassica rapa Botany Genetics Phylogeny Plant Proteins Regulation of gene expression Base Sequence Arabidopsis Proteins fungi Gene Expression Regulation Developmental food and beverages Sequence Analysis DNA General Medicine Plants Genetically Modified biology.organism_classification Gibberellins Dwarfing Plant Leaves Organ Specificity Seedlings Seedling Ectopic expression Gibberellin Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 87:633-643 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-015-0302-5 |
| Popis: | Phytochrome B (phyB) is an essential red light receptor that predominantly mediates seedling de-etiolation, shade-avoidance response, and flowering time. In this study, we isolate a full-length cDNA of PHYB, designated BrPHYB, from Chinese cabbage (Brassica rapa L. ssp. pekinensis), and we find that BrphyB protein has high amino acid sequence similarity and the closest evolutionary relationship to Arabidopsis thaliana phyB (i.e., AtphyB). Quantitative reverse transcription (RT)-PCR results indicate that the BrPHYB gene is ubiquitously expressed in different tissues under all light conditions. Constitutive expression of the BrPHYB gene in A. thaliana significantly enhances seedling de-etiolation under red- and white-light conditions, and causes dwarf stature in mature plants. Unexpectedly, overexpression of BrPHYB in transgenic A. thaliana resulted in reduced expression of gibberellins biosynthesis genes and delayed flowering under short-day conditions, whereas AtPHYB overexpression caused enhanced expression of FLOWERING LOCUS T and earlier flowering. Our results suggest that BrphyB might play an important role in regulating the development of Chinese cabbage. BrphyB and AtphyB have conserved functions during de-etiolation and vegetative plant growth and divergent functions in the regulation of flowering time. |
| Databáze: | OpenAIRE |
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