Detoxified Haemophilus ducreyi cytolethal distending toxin and induction of toxin specific antibodies in the genital tract
| Autor: | Julia Fernandez-Rodrigues, Teresa Lagergård, Karin Ahlman, Annika Lundqvist |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cytolethal distending toxin
medicine.medical_treatment Bacterial Toxins Cell Line Microbiology Haemophilus ducreyi Mice Antibody Specificity medicine Animals Humans Immunity Mucosal Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Pasteurellaceae Public Health Environmental and Occupational Health Toxoid Genitalia Female biology.organism_classification medicine.disease Antibodies Bacterial Antibodies Neutralizing Chancroid Infectious Diseases Immunization Immunoglobulin G Antibody Formation Bacterial Vaccines Immunology biology.protein Molecular Medicine Female Antibody Adjuvant |
| Zdroj: | Vaccine. 28:5768-5773 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2010.06.032 |
| Popis: | Haemophilus ducreyi causes genital ulceration (chancroid), a sexually transmitted infection and still an important factor which contributes to the spread of HIV in developing countries. The bacterium produces a cytolethal distending toxin (HdCDT) causing cell cycle arrest and apoptosis/necrosis of human cells and contributes to the aggravation of ulcers. The aim of the study was to induce toxin-neutralizing antibodies in the genital tract of mice. Repeated subcutaneous (sc) immunisations with 5–10 μg active HdCDT induced low levels of serum anti-HdCDT IgG without neutralizing capacity. High levels of specific IgG1 antibodies in serum and genital tract were generated after sc immunisations with 10 μg formaldehyde detoxified HdCDT toxoid alone and the addition of aluminium salts or RIBI (based on the lipid A moiety) as adjuvant further increased the level of serum antibodies. A high correlation was found between elevated levels of anti-HdCDT IgG in sera, the level of neutralizing activity and the antibody level in genital tract ( r = 0.8). Thus, induction of high antibody levels specific to HdCDT in the genital tissue can be achieved by parenteral immunisation with the toxoid. The HdCDT toxoid can be considered as a candidate component in vaccine against chancroid. |
| Databáze: | OpenAIRE |
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