Evidence that Replication Initiates at Only Some of the Potential Origins in Each Oligomeric Form of Bovine Papillomavirus Type 1 DNA
| Autor: | J B, Schvartzman, S, Adolph, L, Martín-Parras, C L, Schildkraut |
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| Rok vydání: | 1990 |
| Předmět: |
DNA Replication
Binding Sites Restriction Mapping Nucleic Acid Hybridization Cell Biology Chromosomes Cell Line Blotting Southern DNA Viral Animals Electrophoresis Gel Two-Dimensional Electrophoresis Polyacrylamide Gel DNA Circular Papillomaviridae Molecular Biology Bovine papillomavirus 1 Research Article |
| Zdroj: | Molecular and Cellular Biology. 10:3078-3086 |
| ISSN: | 1098-5549 |
| Popis: | In a subclone of ID13 mouse fibroblasts latently infected with bovine papillomavirus type 1 (BPV-1) DNA, the viral genome occurred as a mixture of extrachromosomal circular monomers and oligomers. Multiple copies were also associated with the host cell genome, predominantly at a single site in a head-to-tail tandem array. We examined the replicative intermediates of extrachromosomal forms of BPV-1 DNA by using two-dimensional gel electrophoresis. The results obtained indicate that initiation of DNA replication occurred near the center of the EcoRI-BamHI 5.6-kilobase fragment. In some molecules, however, this fragment was replicated from one end to the other by means of a single fork initiated elsewhere. Termination also occurred within this fragment. The EcoRI-BamHI 2.3-kilobase fragment replicated as a DNA molecule containing a termination site for DNA replication and also by means of a single fork traversing the fragment from one end to the other. Thus, replication forks proceeded through these fragments in different manners, apparently depending on whether they were part of a monomer, a dimer, a trimer, or higher oligomers. These observations lead to the conclusion that initiation of DNA replication in BPV-1 DNA takes place at or close to plasmid maintenance sequence 1. From this point, replication proceeds bidirectionally and termination occurs approximately 180 degrees opposite the origin. The results obtained are consistent with one or more replication origins being quiescent in BPV-1 DNA oligomers. |
| Databáze: | OpenAIRE |
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