Molecular Analysis of Varicella Vaccines and Varicella-Zoster Virus from Vaccine-Related Skin Lesions
| Autor: | Sebastian Voigt, Bernhard Ehlers, Aljona Borschewski, Judit Küchler, Sonja Thiele, Marc Bieberbach |
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| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Herpesvirus 3 Human Varicella vaccine viruses Clinical Biochemistry Immunology Single-nucleotide polymorphism Biology medicine.disease_cause Polymorphism Single Nucleotide Skin Diseases Virus Chickenpox Vaccine medicine Animals Humans Immunology and Allergy SNP Child Adverse effect integumentary system Varicella zoster virus virus diseases Sequence Analysis DNA Exanthema Vaccine Research Virology Open reading frame DNA Viral |
| Zdroj: | Clinical and Vaccine Immunology. 18:1058-1066 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.05021-11 |
| Popis: | To prevent complications that might follow an infection with varicella-zoster virus (VZV), the live attenuated Oka strain (V-Oka) is administered to children in many developed countries. Three vaccine brands (Varivax from Sanofi Pasteur MSD; Varilrix and Priorix-Tetra, both from Glaxo-Smith-Kline) are licensed in Germany and have been associated with both different degrees of vaccine effectiveness and adverse effects. To identify genetic variants in the vaccines that might contribute to rash-associated syndromes, single nucleotide polymorphism (SNP) profiles of variants from the three vaccines and rash-associated vaccine-type VZV from German vaccinees were quantitatively compared by PCR-based pyrosequencing (PSQ). The Varivax vaccine contained an estimated 3-fold higher diversity of VZV variants, with 20% more wild-type (wt) SNPs than Varilrix and Priorix-Tetra. These minor VZV variants in the vaccines were identified by analyzing cloned full-length open reading frame (ORF) orf62 sequences by chain termination sequencing and PSQ. Some of these sequences amplified from vaccine VZV were very similar or identical to those of the rash-associated vaccine-type VZV from vaccinees and were almost exclusively detected in Varivax. Therefore, minorities of rash-associated VZV variants are present in varicella vaccine formulations, and it can be concluded that the analysis of a core set of four SNPs is required as a minimum for a firm diagnostic differentiation of vaccine-type VZV from wt VZV. |
| Databáze: | OpenAIRE |
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