Chironomidae larvae: A neglected enricher of antibiotic resistance genes in the food chain of freshwater environments
| Autor: | Tongdi Yan, Guoqing Cui, Mengmeng Shi, Zhigang Qiu, Junwen Li, Chengshi Ding, Jing Ma, Hanyu Zhao, Wanxiang Jiang, Qi Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Food Chain
010504 meteorology & atmospheric sciences Health Toxicology and Mutagenesis Zoology Fresh Water 010501 environmental sciences Toxicology 01 natural sciences Chironomidae Food chain RNA Ribosomal 16S Water environment Animals Humans Ecosystem 0105 earth and related environmental sciences biology fungi Bacteroidetes Drug Resistance Microbial Aquatic animal General Medicine biology.organism_classification Pollution Anti-Bacterial Agents Genes Bacterial Larva Proteobacteria Bacteria Flavobacterium |
| Zdroj: | Environmental Pollution. 285:117486 |
| ISSN: | 0269-7491 |
| DOI: | 10.1016/j.envpol.2021.117486 |
| Popis: | Infection caused by pathogenic bacteria carrying antibiotic resistance genes (ARGs) is a serious challenge to human health. Water environment, including water and surface sediments, is an important repository of ARGs, and the activity of aquatic animal can affect the development of ARG pollution in the water environment. Macrobenthic invertebrates are an important component of aquatic ecosystems, and their effects on ARG development in aquatic environments remain unreported. The distribution of ARGs, including tetA gene, sul2 gene, and kan gene, in Chironomidae larvae is demonstrated in this study for the first time. The ARG distribution was related to sampling points, metal elements, and seasons. Animal models demonstrated that Chironomidae larvae enriched ARGs from water and passed them on to downstream predators in the food chain. Conjugative transfer mediated by resistant plasmids was crucial in the spread of ARG in Chironomidae larvae, and upregulated expression of trfAp gene and trbBp gene was the molecular mechanism. Escherichia in Proteobacteria and Flavobacterium in Bacteroidetes, which are gram-negative bacteria in Chironomidae larvae, are the primary host bacteria of ARGs confirmed via resistance screening and DNA sequencing of V4 region of 16S rRNA gene. Feeding experiments further confirmed that ARGs from Chironomidae larvae can be enriched in the fish gut. Research gaps in food chain between sediments and fish are addressed in this study, and Chironomidae larvae is an important enricher of ARGs in the freshwater environment. |
| Databáze: | OpenAIRE |
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