Molecular characterization of glutathione peroxidase gene from the liver of silver carp, bighead carp and grass carp
| Autor: | Wei Yao, Guang-Zhao Li, Wan-Qin Liao, Xu-Fang Liang, Wei-Feng Zhu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
China
GPX1 Carps GPX2 5' Flanking Region Molecular Sequence Data Biochemistry Gene Expression Regulation Enzymologic Botany Animals Amino Acid Sequence RNA Messenger Cloning Molecular Carp Molecular Biology Phylogeny chemistry.chemical_classification Glutathione Peroxidase Silver carp Hypophthalmichthys Base Sequence Sequence Homology Amino Acid biology Gene Expression Profiling Glutathione peroxidase Sequence Analysis DNA General Medicine biology.organism_classification Bighead carp Grass carp Liver chemistry sense organs |
| Zdroj: | BMB Reports. 41:204-209 |
| ISSN: | 1976-6696 |
| DOI: | 10.5483/bmbrep.2008.41.3.204 |
| Popis: | The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5`-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins. |
| Databáze: | OpenAIRE |
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