Transient Callosal Projections of L4 Neurons Are Eliminated for the Acquisition of Local Connectivity

Autor: Maria J. Galazo, Ignacio Varela, Linnea A. Weiss, Gertrudis Perea, N. S. De León Reyes, S. Mederos, Marta Nieto
Přispěvatelé: León, Reyes N.S. De, Mederos, S., Weiss, L.A., Galazo, M.J., Nieto, M., León, Reyes N.S. De [0000-0002-4070-5983], Mederos, S. [0000-0003-0960-9974], Weiss, L.A. [0000-0002-7593-7821], Galazo, M.J. [0000-0003-1909-7765], Nieto, M. [0000-0002-8349-8435]
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Sensory Receptor Cells
Science
Green Fluorescent Proteins
General Physics and Astronomy
Mice
Transgenic
Sensory system
Biology
Somatosensory system
Corpus callosum
Article
General Biochemistry
Genetics and Molecular Biology
Corpus Callosum
03 medical and health sciences
0302 clinical medicine
Thalamus
Neural Pathways
medicine
Animals
Transient (computer programming)
lcsh:Science
Cell fate and cell lineage
Mice
Knockout
Neurons
Microscopy
Confocal
Multidisciplinary
Genetic interventions
Axon and dendritic guidance
Cell type diversity
Development of the nervous system
Somatosensory Cortex
General Chemistry
Axons
Mice
Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Animals
Newborn
nervous system
Cerebral cortex
Excitatory postsynaptic potential
lcsh:Q
Neuron
Neuroscience
030217 neurology & neurosurgery
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
Nature Communications
Popis: Interhemispheric axons of the corpus callosum (CC) facilitate the higher order functions of the cerebral cortex. According to current views, callosal and non-callosal fates are determined early after a neuron's birth, and certain populations, such as cortical layer (L) 4 excitatory neurons of the primary somatosensory (S1) barrel, project only ipsilaterally. Using a novel axonal-retrotracing strategy and GFP-targeted visualization of Rorb+ neurons, we instead demonstrate that L4 neurons develop transient interhemispheric axons. Locally restricted L4 connectivity emerges when exuberant contralateral axons are refined in an area- and layer-specific manner during postnatal development. Surgical and genetic interventions of sensory circuits demonstrate that refinement rates depend on distinct inputs from sensory-specific thalamic nuclei. Reductions in input-dependent refinement result in mature functional interhemispheric hyperconnectivity, demonstrating the plasticity and bona fide callosal potential of L4 neurons. Thus, L4 neurons discard alternative interhemispheric circuits as instructed by thalamic input. This may ensure optimal wiring.
This work was funded by grants from MINECO SAF2014-52119-R, BFU2016-81887-REDT, PCIN-2015-176-C02-02/ERA-Net Neuron (Era-Net,MINECO), MCIU/AEI/FEDER, UE SAF2017-83117-R.
Databáze: OpenAIRE
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