Biological performance of mussel-inspired adhesive in extrahepatic islet transplantation
| Autor: | Phillip B. Messersmith, Ling-Jia Wang, Dixon B. Kaufman, Carrie E. Brubaker, Hermann Kissler |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty Materials science Biocompatibility Islets of Langerhans Transplantation Biophysics Bioengineering Article Diabetes Mellitus Experimental Polyethylene Glycols Biomaterials Mice Biomimetic Materials Materials Testing PEG ratio medicine Animals Humans geography geography.geographical_feature_category Molecular Structure Biomaterial Hydrogels Glucose Tolerance Test Islet Bivalvia Surgery Cell biology Mice Inbred C57BL Transplantation Mechanics of Materials Self-healing hydrogels Ceramics and Composites Chronic inflammatory response Tissue Adhesives Adhesive |
| Zdroj: | Biomaterials. 31:420-427 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2009.09.062 |
| Popis: | There is significant need for effective medical adhesives that function reliably on wet tissue surfaces with minimal inflammatory insult. To address these performance characteristics, we have generated a synthetic adhesive biomaterial inspired by the protein glues of marine mussels. In-vivo performance was interrogated in a murine model of extrahepatic syngeneic islet transplantation, as an alternative to standard portal administration. The adhesive precursor polymer consisted of a branched poly(ethylene glycol) (PEG) core, whose endgroups were derivatized with catechol, a functional group abundant in mussel adhesive proteins. Under oxidizing conditions, adhesive hydrogels formed in less than one minute from catechol-derivatized PEG (cPEG) solutions. Upon implantation, the cPEG adhesive elicited minimal acute or chronic inflammatory response in C57BL6 mice, and maintained an intact interface with supporting tissue for up to one year. In-situ cPEG adhesive formation was shown to efficiently immobilize transplanted islets at the epididymal fat pad and external liver surfaces, permitting normoglycemic recovery and graft revascularization. These findings establish the use of synthetic, biologically-inspired adhesives for islet transplantation at extrahepatic sites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect