acal is a long non-coding RNA in JNK signaling in epithelial shape changes during Drosophila dorsal closure
| Autor: | Luis Daniel Rios-Barrera, Maria Dominguez, Juan R. Riesgo-Escovar, Irene Gutierrez-Perez |
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| Přispěvatelé: | Universidad Nacional Autónoma de México, Consejo Nacional de Ciencia y Tecnología (México), Ministerio de Economía y Competitividad (España), Generalitat Valenciana, Fundación Botín |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Regulation of gene expression
Scaffold protein Cancer Research lcsh:QH426-470 Drosophila embryogenesis Biology Molecular biology Dorsal closure lcsh:Genetics AP-1 transcription factor Gene expression Genetics Molecular Biology Transcription factor Genetics (clinical) Ecology Evolution Behavior and Systematics Drosophila Protein Research Article |
| Zdroj: | PLoS Genetics, Vol 11, Iss 2, p e1004927 (2015) PLoS Genetics |
| Popis: | Dorsal closure is an epithelial remodeling process taking place during Drosophila embryogenesis. JNK signaling coordinates dorsal closure. We identify and characterize acal as a novel negative dorsal closure regulator. acal represents a new level of JNK regulation. The acal locus codes for a conserved, long, non-coding, nuclear RNA. Long non-coding RNAs are an abundant and diverse class of gene regulators. Mutations in acal are lethal. acal mRNA expression is dynamic and is processed into a collection of 50 to 120 bp fragments. We show that acal lies downstream of raw, a pioneer protein, helping explain part of raw functions, and interacts genetically with Polycomb. acal functions in trans regulating mRNA expression of two genes involved in JNK signaling and dorsal closure: Connector of kinase to AP1 (Cka) and anterior open (aop). Cka is a conserved scaffold protein that brings together JNK and Jun, and aop is a transcription factor. Misregulation of Cka and aop can account for dorsal closure phenotypes in acal mutants. LDRB is a doctoral student from Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México and received fellowship #229328 from Consejo Nacional de Ciencia y Tecnología (http://www.conacyt.gob.mx). Laboratory funding was provided by Consejo Nacional de Ciencia y Tecnología grant #177962, Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica de la Universidad Nacional Autónoma de México (http://dgapa.unam.mx/html/papiit/papit.html), grant #IN203110, and laboratory budget to JRRE. MD is a grant recipient form the Spanish National Grants (BFU2009-09074, SAF2012-35181 and MEC-CONSOLIDER CSD2007-00023), Generalitat Valenciana Grant (PROMETEO II/2013/001) and Fundación Botín. IGP is a grant recipient from the IFP, Spain. |
| Databáze: | OpenAIRE |
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