A novel gene from Myxococcus xanthus that facilitates membrane translocation of an extracellular endoglucanase in Escherichia coli?
| Autor: | Chantal Monnier, Janine Guespin-Michel, Laila Bensmail, Laurent Quillet, Sylvie Barray |
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| Rok vydání: | 2001 |
| Předmět: |
Signal peptide
Myxococcus xanthus Molecular Sequence Data Protein Sorting Signals medicine.disease_cause Microbiology Gene Expression Regulation Enzymologic Open Reading Frames Cellulase Escherichia coli medicine Extracellular Amino Acid Sequence Cloning Molecular Molecular Biology Gene biology fungi Membrane Proteins General Medicine Periplasmic space biology.organism_classification Open reading frame Biochemistry Cytoplasm bacteria Plasmids |
| Zdroj: | Research in Microbiology. 152:487-492 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/s0923-2508(01)01222-0 |
| Popis: | Expression in Escherichia coli of the Myxococcus xanthus gene celA, which encodes an extracellular endoglucanase, resulted in CelA being distributed between cytoplasm, periplasm and membrane. The presence of an adjacent open reading frame downstream from the full celA gene, or the absence of a putative lipoprotein signal sequence, confined CelA distribution to the periplasm and membrane, or to the cytoplasm and periplasm, respectively. |
| Databáze: | OpenAIRE |
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