Evolutionary relationships between yeast and bacterial homoserine dehydrogenases
| Autor: | Dominique Thomas, Yolande Surdin-Kerjan, Régine Barbey |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Gram-negative bacteria
Evolution of enzyme function Gram-positive bacteria Genes Fungal Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Biophysics Homoserine medicine.disease_cause Biochemistry Microbiology chemistry.chemical_compound Structural Biology Escherichia coli Genetics medicine Homoserine dehydrogenase Amino Acid Sequence Cloning Molecular Molecular Biology Base Sequence Sequence Homology Amino Acid biology Cell Biology biology.organism_classification Biological Evolution Yeast chemistry Genes Bacterial Bacteria |
| Zdroj: | FEBS Letters. 323:289-293 |
| ISSN: | 0014-5793 |
| Popis: | The Saccharomyces cerevisiae HOM6 gene, encoding homoserine dehydrogenase (EC 1.1.1.3) was cloned and its nucleotide sequence determined. The yeast homoserine dehydrogenase shows extensive homology to the homoserine dehydrogenase domains of the two aspartokinase-homoserine dehydrogenases from Escherichia coli as well as to the homoserine dehydrogenases from Gram positive bacteria. Sequence alignment reveals that the yeast enzyme is the smallest homoserine dehydrogenase known, owing to the absence of a C-terminal domain endowed with the l-threonine allosteric response in Gram positive bacteria. Accordingly, the S. cerevisiae enzyme appears to be a naturally occurring feedback resistant homoserine dehydrogenase. Our results indicate that homoserine dehydrogenase was originally an unregulated enzyme and that feedback control acquisition occured twice during evolution after the divergence between Gram positive and Gram negative bacteria. |
| Databáze: | OpenAIRE |
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